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| Effects of Filamentous Alga Chaetomorpha valida Degradation on Aquaculture Water Quality, and Growth and Immunity of Sea Cucumber Apostichopus japonicus |
| WANG Xin1, XING Ronglian1, ZHANG Hongxia1, LIU Chuyao1, WANG Linqian1, LIU Renyu1, CHEN Lihong1, XU Xiaoguang2, CAO Xuebin3, CHENG Cao4, SU Qun5 |
1. Yantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, School of Life Sciences, Yantai University, Yantai 264005, China;
2. Cooperation and Operations Division of the Science and Education Park Management Office, Yantai University, Yantai 264005, China;
3. Yantai Jinghai Marine Fisheries Co., Ltd, Yantai, Shandong, Yantai 264006, China;
4. Anyuan Marine Breeding Co., Ltd, Yantai, Shandong, Yantai 264006, China;
5. Baoyuan Bio-Agri Science & Technology (Shandong) Co., Ltd, Yantai Shandong, Yantai 264006, China |
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Abstract To investigate the ecological effects of the degradation of filamentous alga Chaetomorpha valida on the growth and immune response of sea cucumbers Apostichopus japonicus, an indoor cultivation experiment imitating sea cucumber was conducted. sea cucumber were reared in natural seawater (control group) and 150 g (wet weight) of frozen death filamentous alga C. valida (alga treatment group) for 20 d at water temperature of (17.0±0.5) ℃. The immune indices including activities of superoxide dismutase, catalase, acid phosphatase, alkaline phosphatase, and total antioxidant capacity and malondialdehyde contents were determined in the body wall and intestine of the sea cucumber as well as the physicochemical indices in water on the 0th, 5th, 10th, 15th and 20th days of the culture. The resulted showed that the concentrations of ammonia nitrogen and nitrite nitrogen were significantly elevated in the alga treatment group compared to the control group (P<0.05) during the C. valida degradation process, superoxide dismutase and catalase activities and total antioxidant capacity were shown significantly higher in the body wall and intestine than that in the control group from 0 d to 10 d in degradation (P<0.05). The activities of superoxide dismutase and catalase showed a decreasing trend, and both activities were significantly lower in the algal treatment group than those in the control group(P<0.05) from 15 d to 20 d. During the degradation process of C. valida, the sea cucumber had growth rate of 0.18%/d, significantly lower than the sea cucumber in the control group did (P<0.05). The levels of malondialdehyde were found to be gradually increased in the body wall and intestines of sea cucumber. The ecological effects caused by the degradation of C. valida led to reduce the growth rate of sea cucumber and to damage their immune system. The findings provide new theoretical support and basis for the healthy breeding and scientific management of A. japonicus.
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Received: 24 August 2024
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