脯氨酸对中华鳖受损裙边细胞生长及胶原代谢的影响

doi:10.16378/j.cnki.1003-1111.24195

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Abstract Proline (Pro) is a conditionally essential amino acid in animals, with important nutritional, metabolic, and immune functions. To investigate the effect and mechanism of proline on oxidative stress in vitro in fibroblastic cells from the embryonic calipash of Chinese soft-shelled turtle Pelodiscus sinensis, a lipopolysaccharide induced oxidative stress model was established in the experiment. The cells in the cell culture system were divided into control, LPS stressed, and Pro treated groups. The cell viability, reactive oxygen species (ROS) content, and mRNA expression levels of collagen metabolism related genes were determined by MTT, HE staining, DCFH-DA, and qRT-PCR. The results showed that there were significant decrease in the number and survival rate of cells, significant increase in ROS levels, and a remarkable reduction in the mRNA expression abundance of collagen synthesis related genes Col1α1, Col1α2, and Col3α1 (P<0.05) in the LPS stressed group compared with the control, with significant escalation in the mRNA expression of collagen degradation related genes MMP1 and MMP18 (P<0.05). Correspondingly, the mRNA levels of MMPs specific inhibitors TIMP2, TIMP3, and TIMP4, key enzymes for collagen degradation were found to be significantly declined (P<0.05). The supplementation of Pro led to significantly elevate the number and survival rate of stressed cells (P<0.05), and significantly reduce the content of ROS (P<0.05) compared with the LPS group. Significantly up-regulated relative mRNA expression levels of Col3α1, TIMP2 and TIMP3, and significantly down-regulated mRNA expression levels of MMP18 (P<0.05, P<0.01) were shown to be in the fibroblastic cells from the embryonic calipash of Chinese soft-shelled turtle treated with Pro, indicating that Pro can alleviate LPS induced oxidative stress, in fibroblastic cells of Chinese soft-shelled turtle, possibly by altering the ROS signaling pathway, regulating the expression of collagen metabolism related enzymes MMPs and their inhibitory factors TIMPs. The findings revealed the effects of Pro on the growth and collagen metabolism of LPS induced damage in the fibroblastic cells from the embryonic calipash of Chinese soft-shelled turtle, which can provide theoretical basis for the application of similar functional amino acids such as Pro to enhance the stress resistance of Chinese soft-shelled turtle.

Key words： proline lipopolysaccharide stress collagen metabolism calipash Pelodiscus sinensis

Received: 15 November 2024

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S963

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	ZHU Leyan
	ZHOU Beining
	SONG Wei
	LUO Jiaxiang
	LI Caiyan

Cite this article:

ZHU Leyan,ZHOU Beining,SONG Wei, et al. Effect of Proline on Growth and Collagen Metabolism in the Injured Calipash Cells of Chinese Soft-Shelled Turtle Pelodiscus sinensis[J]. Fisheries Science, 2025, 44(4): 642-650.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24195 OR https://www.shchkx.com/EN/Y2025/V44/I4/642

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