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| Optimization and Protective Effect of Complex Protectant for Rhodobacter Used in Aquaculture |
| CAO Haipeng1,2, ZHANG Xiuxian1,2, ZHENG Xurui1,2, SONG Xincheng3, WANG Chao3, AN Jian3 |
1. National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China;
2. Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China;
3. Lianyungang Marine and Fisheries Development Promotion Center, Lianyungang 222000, China |
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Abstract To develop a complex protectant for Rhodobacter used in aquaculture and evaluate its protective effect on Rhodobacter, single excellent protectants for Rhodobacter were screened from 13 kinds of protectants by single-factor method using R. azotoformans SY5 as the screening indicator bacterium. The composite ratio of the screened single excellent protectants was further optimized by orthogonal tests to develop the complex protectant. Besides, the protective effects of the complex protectant on the illumination stability, storage survival rate and immunomodulatory property of Rhodobacter were analyzed for Chinese mitten crab Eriocheir sinensis. The results showed that glucose, sucrose, mannitol, and trehalose were excellent protectants for Rhodobacter, with the optimal ratio of 12.5 g∶17.5 g∶12.5 g∶17.5 g. The resultant complex protectant developed under the optimal composite ratio led to enhance the illumination stability and storage survival rate of Rhodobacter at the optimal dose of 0.30 g/mL, to increase the 10-day survival rate of R. azotoformans SY5 at an illumination intensity of 4500 lx by 28.67 percentage point(P<0.05), and to enhance the 40-day storage survival rate of R. azotoformans SY5 by 72.83 percentage point(P<0.05). In addition, the serum activities of the R. azotoformans SY5 were found to be elevated from 0.32% (P>0.05) to 2.63% (P<0.05) in acid phosphatase, from 0.54% (P>0.05) to 3.91% (P<0.05) in superoxide dismutase, from 0.32% (P>0.05) to 3.22% (P>0.05) in catalase, from 1.07% (P>0.05) to 7.39% (P<0.05) in hepatopancreatic acid phosphatase, from 0.79% (P<0.05) to 2.09% (P<0.05) in superoxide dismutase and from 0.08% (P>0.05) to 2.13% (P>0.05) in catalase in the induced Chinese mitten crab by the dietary complex protectant, from 3.0 to 9.0 g/kg. The findings confirm the complex protectant as an excellent protectant for Rhodobacter with the ability to improve the illumination stability, storage survival rate and immunomodulatory performance, and can lay a foundation for the setup of the complex protectant based stability enhancement technology of Rhodobacter used in aquaculture.
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Received: 19 March 2025
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