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| Bacterial Diversity of "Exophthalmia" in Largemouth Bass Micropterus salmoides Based on 16S rRNA Sequencing |
| HANG Yitong1,2, LI Bingbu1,2, HE Zhongwei1,2, LIU Yufeng1,2, CAO Wei1,2, HAN Tian1,2, HE Ziyang1,2, WANG Guixing1,2, REN Yuqin1,2, GONG Chunguang3, HOU Jilun1,2 |
1. Hebei Key Laboratory of the Bohai Sea Fish Germplasm Resources Conservation and Utilization, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China;
2. Bohai Sea Fishery Research Center, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China;
3. Ocean College, Hebei Agricultural University, Qinhuangdao 066009, China |
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Abstract In order to elucidate the shifts in bacterial community structure in diseased cultured largemouth bass (Micropterus salmoides) in an outbreak of "exophthalmia" which was featured by bilateral ocular protrusion with opaque corneal membranes and suffered >90% of mortality in Tangshan, Hebei Province, February 2025, the dominant bacterial taxa was identified and the bacterial flora structure and diversity in the eyes and internal organs (excluding the intestine) were compared between the diseased and healthy fish using 16S rRNA high-throughput sequencing. Pseudomonadota was found to be dominant in diseased eyes, with decrease markedly (72.71% vs 87.50%—95.34% in healthy controls), and significant increase in Actinomycetota, Bacillota, and Bacteroidota at the phylum level. Paracoccus (0.997% vs 0.136%), Dietzia (1.293% vs 0.045%), Leucobacter (0.886% vs 0.062%), Glutamicibacter (1.057% vs 0.009%),Mammaliicoccus (2.320% vs 0.020%), Chryseobacterium (2.710% vs 0.373%), and Sphingobacterium (0.969% vs 0.057%) were showed to be selectively enriched in diseased eyes at the genus level. It was found that the exophthalmia in largemouth bass was likely driven by a polymicrobial consortium rather than a single pathogen, which provides critical insights for accurate diagnosis, prevention, and targeted control strategies.
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Received: 15 May 2025
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