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| Effects of Salinity Stress on Activities of Enzymes Related to Immune of Snail Babylonia areolata |
| TAN Chunming1,2, ZHAO Wang1,2, WU Kaichang1,2, WEN Weigeng1,2, YANG Rui1, CHEN Xu1,2, YU Gang1,2 |
1. Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China;
2. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China |
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Abstract Behavior and activities of six enzymes related to immune were determined in juvenile snail Babylonia areolata with average body weight of (0.386±0.080) g exposed to a salinity of 18, 23, 28, 33 (control group) and 38 at water temperature of (26.0±1.0) ℃ for 6, 12, 24, 36, 48, 72 and 96 hours to evaluate the changes in activities of the enzymes. The results showed that the suitable salinity for growth of the snail was ranged from 20 to 35, and high mortality was observed at the salinity of below 15 or above 45. Both the increase in salt concentration and in exposure period led to have significant effects on the activities of the immune enzymes (P<0.05). Compared with the control group, the catalase activity showed a “suppression-induction” trend in the snail in salinity 18 and 23 groups, and an “induction-suppression-induction” in the salinity 28 and 38 groups. The consistent “induction-suppression” in acid phosphatase and alkaline phosphatase activities were found in the snail in each salinity group, and the same case was observed in total superoxide dismutase activity of the snail in salinity 38 group, in catalase activity in the same salinity group. The total superoxide dismutase activity showed the trend of “induction-suppression” in salinity 18, 23 and 28 groups. The trend of “suppression-induction-suppression” in glutathione peroxidase activity in each salinity group and the peroxidase activity in the salinity 18 and 38 groups were found. The peroxidase activity in salinity 23 and 28 groups was consistent with the total superoxide dismutase activity change in the same salinity. So, the salinity in the aquaculture water will have a greater impact on the growth and immune enzyme activity of the snail. The catalase, alkaline phosphatase, total superoxide dismutase, acid phosphatase and glutathione peroxidase activities were induced when B. areolata were subjected to mild stress, while their activities were inhibited by severe stress.
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Received: 05 May 2019
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