一株耐盐硅藻杯状藻的生长和生化组成分析

doi:10.16378/j.cnki.1003-1111.20142

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Abstract The naviculoid genus Craticula was widely distributed in alkaline saline waters. The halotolerant diatom strain Craticula sp. FACHB-2450 was isolated from saline lake in this work. The present study evaluated the effect of different salinities on growth, polysaccharides, soluble proteins, lipids and fatty acid profiles of strain FACHB-2450, as well as the photosynthetic physiological response under laboratory conditions. It was found that the maximal biomass concentration cultured was 1.79 g/L at 6% NaCl . The F_v/F_m value was increased up to 0.63 at 3% NaCl then decreased at higher salinity, with the maximal polysaccharides content cultured of 9.96% of dry weight at 6% NaCl. The soluble protein content was decreased from 22.78% to 23.65% of dry weight at 1.5%—9% NaCl sharply to 8.39% of dry weight at 12% NaCl. The maximal total lipid content cultured at 3% NaCl was 32.46% of dry weight. The main fatty acid components of strain FACHB-2450 were palmitoleic acid (C16:1) (30.17%—43.75% of total fatty acid), palmitic acid (C16:0) (22.53%—31.45% of total fatty acid), and eicosapentaenoic acid (EPA, C20:5) (14.79%—20.92% of total fatty acid). The content of the most abundant unsaturated fatty acid C20:5 was increased at lower salinity then decreased at higher salinity with the maximal concentration at 9% NaCl.

Key words： diatom Craticula sp. NaCl growth biochemical composition

Received: 02 July 2020

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	Articles by authors
	ZHANG Qi
	ZHENG Lingling
	LI Tianli
	LIU Jin
	SONG Lirong

Cite this article:

ZHANG Qi,ZHENG Lingling,LI Tianli, et al. Growth and Biochemical Composition of a Halotolerant Diatom Strain of Craticula sp.[J]. Fisheries Science, 2022, 41(2): 250-257.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20142 OR https://www.shchkx.com/EN/Y2022/V41/I2/250

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