Testis Development Induced in Japanese Eel Anguilla japonica by 17α-Methyltestosterone Exposure
LAI Xiaojian1,2, PENG Shuai1, ZHANG Zhe3, LI Zhongqin1,2
1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Engineering Research Center of the Modern Industry Technology for Eel, Ministry of Education, Xiamen 361021, China; 3. Fisheries Research Institute of Fujian, Xiamen 361000, China
Abstract：The male Japanese eel Anguilla japonica with body weight of (266.45±18.65) g was held in a round culture bucket with a diameter of 1.5 m, a height of 1.0 m and a water volume of 1000 L at a rate of 10 fish and exposed to 17α-methyltestosterone concentration of 50 μg/L and to 5 mL of absolute ethanol in the control group at water temperature of (21±1) ℃ and a salinity of 35 for 45 d, without feeding, and with half exchange of the water every 5 days. After 17α-methyltestosterone exposure, the histological sections of Japanese eel testis were examined and serum 11-ketotestosterone (11-KT) and estradio (E2) levels were determined by ELISA to investigate the promoting effect of 17α-methyltestosterone on gonadal development and possible mechanism. The results showed that the male Japanese eel exposed to 17α-methyltestosterone concentration, with white and flaky testis at phase Ⅳ/ spermiogenesis stage (S5), had gonado-somatic index of (1.63±0.68)%, significantly higher than the eel in the control (about 8 folds) did, with thin testis with serrated edge at phase Ⅱ/ spermatogonial proliferation stage (S1) in the controls group. There was significantly higher serum E2 level in the male Japanese eel exposed to 17α-methyltestosterone [(182.06±30.52) pg/mL] than that in the control group (P<0.05), without significant change in serum 11-KT level between the male Japanese eel exposed to 17α-methyltestosterone [(7.30±2.15) pg/mL] and male Japanese eel in the control group, indicating that MT might induced spermatogenesis in Japanese eel by exposure.
赖晓健, 彭帅, 张哲, 李忠琴. 甲基睾酮暴露对鳗鲡精巢发育的影响[J]. 水产科学, 2021, 40(6): 900-904.
LAI Xiaojian, PENG Shuai, ZHANG Zhe, LI Zhongqin. Testis Development Induced in Japanese Eel Anguilla japonica by 17α-Methyltestosterone Exposure. Fisheries Science, 2021, 40(6): 900-904.
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2021, Vol. 40 
