Effects of Dietary Methyltestosterone on Growth and Body Pigmentation of Female Sciaenochromis fryeri
JIANG Jufeng1, LIU Keming1, LI Chunyan1, LIU Xiaolian1, ZHOU Yong1, HAN Xianqin1, SHI Dongjie2, MA Lin1, SONG Hongmei3, ZHAO Liming4
1. Tianjin Ornamental Fish Technology and Engineering Center, Tianjin Fisheries Research Institute, Tianjin 300221, China; 2. National Engineering Research Center for Freshwater Fisheries, Beijing Fisheries Research Institute, Beijing 100068, China; 3. Key Laboratory of Recreational Fisheries, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; 4. Development Services Center of Animal Husbandry and Fisheries of Jinghai District, Tianjin 301600, China
Abstract：In order to study effects of methyltestosterone on growth and body colour in female Sciaenochromis fryeri, 450 individuals of female S. fryeri with initial body weight of (5.78±1.05) g were randomly divided into 5 groups and fed diets containing methyltestosterone at a dose of 0 (control group), 4, 8, 12, and 16 mg/kg for 8 weeks, and then all fishes were fed the control diet for 4 weeks. Growth performance and body pigmentation of female S. fryeri were determined every 2 weeks during the 12 weeks experimental period. The results showed that there was significantly higher average body weight in the female S. fryeri in 12 mg/kg group than that in the other groups (P<0.05), even though with 100% of average survival rate in each group. There was no significantly difference in body pigmentation between 12 mg/kg group and 16 mg/kg group(P>0.05), significantly higher than that in the other groups (P<0.05). The fish colour was all elevated somewhat in all groups, and then decreased slightly, being remained at stable level constantly after the colouration of female S. fryeri for 8 weeks was enhanced. The findings indicated that the methyltestosterone had adaptive reaction to female S. fryeri, and that the optimal methyltestosterone dose was of 12 mg/kg.
姜巨峰, 刘克明, 李春艳, 刘肖莲, 周勇, 韩现芹, 史东杰, 马林, 宋红梅, 赵立明. 饲料中甲基睾酮含量对阿里雌鱼生长及体色的影响[J]. 水产科学, 2022, 41(3): 445-451.
JIANG Jufeng, LIU Keming, LI Chunyan, LIU Xiaolian, ZHOU Yong, HAN Xianqin, SHI Dongjie, MA Lin, SONG Hongmei, ZHAO Liming. Effects of Dietary Methyltestosterone on Growth and Body Pigmentation of Female Sciaenochromis fryeri. Fisheries Science, 2022, 41(3): 445-451.
[1] 喻翔.虾青素与甲基睾丸酮对樱桃鲃生长与体色的影响[D].西安:西北大学,2012:4-6. [2] CAN E, KIZAK V, SEYHANEYILDIZ CAN Ş, et al. Anesthetic potential of Geranium (Pelargonium graveolens) oil for two cichlid species, Sciaenochromis fryeri and Labidochromis caeruleus[J].Aquaculture,2018,491:59-64. [3] MORADIAN A M, DORAFSHAN S, PAYKAN HEYRATI F, et al. Effects of dietary bovine lactoferrin on growth, haemato-biochemical parameters, immune functions and tolerance to air exposure stress in the African cichlid Sciaenochromis fryeri[J].Aquaculture Nutrition,2018,24(1):392-399. [4] KENAN G, DERYA G, IHSAN C, et al. Optimal dietary protein levels in juvenile electric blue cichlid (Sciaenochromis fryeri)[J].The Israeli Journal of Aquaculture-Bamidgeh, 2008,60(4): 261-267. [5] 城市渔夫,快枪手,KONINGS A D,等.马拉维湖慈鲷点将录(3) 阿里[J].水族世界,2005(3):60-63. [6] 林家豪,陈睿毅,楼宝,等.17α-甲睾酮对黄姑鱼幼鱼生长及性分化的影响[J].浙江海洋学院学报(自然科学版),2015,34(1):20-25. [7] 刘少贞,吕文琪,吕小也.17α-甲基睾酮对斑马鱼肝脏vtg基因mRNA表达的影响[J].畜牧与饲料科学,2016,37(5):9-11. [8] 姚汶励,姜鹏,白俊杰.17α-甲基睾酮对草鱼性腺发育及性类固醇激素水平的影响[J].水产学报,2019,43(4):801-809. [9] 韩建,许言,余逸敏,等.17α-甲基睾酮对剑尾鱼的影响[J].中国实验动物学报,2010,18(6):484-488. [10] 周兴华,向枭,王进文.甲基睾酮对珍珠玛丽鱼及红剑尾鱼性逆转及体色影响的初步研究[J].水产科技情报,2000:99-101. [11] 陈小江,王建国,王权.甲基睾酮对中华鳑鲏体色的影响[J].江苏农业科学,2017,45(22):192-194. [12] 黄海,张希,杨宁.应用甲基睾丸酮诱导马拉瓜丽体鱼雄性化的研究[J].热带农业科学,2012,32(9):68-71. [13] LONE K P, MATTY A J. The effect of feeding methyltestosterone on the growth and body composition of common carp (Cyprinus carpio L.)[J].General and Comparative Endocrinology,1980,40(4):409-424. [14] 姜巨峰,张殿昌,邱丽华,等.用IGF-ⅠmRNA表达量评价鲮饲料配方效果的研究[J].南方水产,2010,6(2):66-72. [15] 谭芸,朱佳杰,周宇,等.温度诱导及药物处理对吉富罗非鱼雄性率及生长发育的影响[J].大连海洋大学学报,2015,30(3):253-256. [16] BLÁZQUEZ M, ZANUY S, CARRILLO M, et al. Structural and functional effects of early exposure to estradiol-17β and 17α-ethynylestradiol on the gonads of the gonochoristic teleost Dicentrarchus labrax[J].Fish Physiology and Biochemistry,1998,18(1):37-47. [17] 李家乐,李思发,韩风进.甲基睾丸酮诱导吉富品系尼罗非鲫雄性化的研究[J].水产学报,1997,21(增刊):107-110. [18] HENDRY C I, MARTIN-ROBICHAUD D J, BENFEY T J. Hormonal sex reversal of Atlantic halibut (Hippoglossus hippoglossus L.)[J].Aquaculture,2003,219(1/2/3/4):769-781. [19] 周家辉,杜金星,姜鹏,等.17α-甲基睾酮对大口黑鲈生长及性腺发育的影响[J].中国水产科学,2021,28(9):1109-1117. [20] 刘阿朋,查金苗,王子健,等.17α-甲基睾酮对稀有鮈鲫幼鱼性腺发育与血清卵黄蛋白原水平的影响[J].生态毒理学报,2006,1(3):254-258. [21] 范俊杰,徐少群,方展强,等.17α-甲基睾酮对食蚊鱼形态雄性化及目标基因表达的影响[J].水产学报,2013,37(1):9-15. [22] 李飞,胡琼,张俊杰.17α-甲基睾丸酮诱导白斑狗鱼性逆转初步研究[J].淡水渔业,2018,48(1):3-8. [23] KNÖRR S, BRAUNBECK T. Decline in reproductive success, sex reversal, and developmental alterations in Japanese medaka (Oryzias latipes) after continuous exposure to octylphenol[J].Ecotoxicology and Environmental Safety,2002,51(3):187-196. [24] 李云,李英文,向枭,等.类胡萝卜素甲基睾酮对观赏鱼体色的影响[J].西南农业大学学报,1999,21(3):270-273. [25] 郑曙明,陈章宝.甲基睾酮对孔雀鱼、玛丽鱼性逆转的研究[J].四川畜牧兽医学院学报,1998,12(2):4-8. [26] 黄永政.17α-甲基睾酮与类胡萝卜素对观赏鱼体色影响的研究[D].重庆:西南大学,2008:21. [27] 邹琴,陈培基,李刘冬,等.甲基睾丸酮在罗非鱼苗种体内的消解规律[J].水生生物学报,2010,34(2):250-255. [28] JOHNSTONE R, MACINTOSH D J, WRIGHT R S. Elimination of orally administered 17α-methyltestosterone by Oreochromis mossambicus (tilapia) and Salmo gairdneri (rainbow trout) juveniles[J].Aquaculture,1983,35:249-257. [29] 祝璟琳,邹芝英,杨弘,等.甲基睾丸酮在尼罗罗非鱼(Oreochromis niloticus)肌肉中的代谢和消除规律研究[J].海洋与湖沼,2012,43(5):1016-1022. [30] GULLU K, GUZEL S, EKICI K, et al. Residue analysis and effects of 17α-methyltestosterone on body composition of rainbow trout, Oncorhynchus mykiss[J].Italian Journal of Food Science,2007,19(3):357-362.
2022, Vol. 41 
