Testis Development Induced in Japanese Eel Anguilla japonica by 17α-Methyltestosterone Exposure
LAI Xiaojian1,2, PENG Shuai1, ZHANG Zhe3, LI Zhongqin1,2
1. Fisheries College, Jimei University, Xiamen 361021, China; 2. Engineering Research Center of the Modern Industry Technology for Eel, Ministry of Education, Xiamen 361021, China; 3. Fisheries Research Institute of Fujian, Xiamen 361000, China
Abstract：The male Japanese eel Anguilla japonica with body weight of (266.45±18.65) g was held in a round culture bucket with a diameter of 1.5 m, a height of 1.0 m and a water volume of 1000 L at a rate of 10 fish and exposed to 17α-methyltestosterone concentration of 50 μg/L and to 5 mL of absolute ethanol in the control group at water temperature of (21±1) ℃ and a salinity of 35 for 45 d, without feeding, and with half exchange of the water every 5 days. After 17α-methyltestosterone exposure, the histological sections of Japanese eel testis were examined and serum 11-ketotestosterone (11-KT) and estradio (E2) levels were determined by ELISA to investigate the promoting effect of 17α-methyltestosterone on gonadal development and possible mechanism. The results showed that the male Japanese eel exposed to 17α-methyltestosterone concentration, with white and flaky testis at phase Ⅳ/ spermiogenesis stage (S5), had gonado-somatic index of (1.63±0.68)%, significantly higher than the eel in the control (about 8 folds) did, with thin testis with serrated edge at phase Ⅱ/ spermatogonial proliferation stage (S1) in the controls group. There was significantly higher serum E2 level in the male Japanese eel exposed to 17α-methyltestosterone [(182.06±30.52) pg/mL] than that in the control group (P<0.05), without significant change in serum 11-KT level between the male Japanese eel exposed to 17α-methyltestosterone [(7.30±2.15) pg/mL] and male Japanese eel in the control group, indicating that MT might induced spermatogenesis in Japanese eel by exposure.
赖晓健, 彭帅, 张哲, 李忠琴. 甲基睾酮暴露对鳗鲡精巢发育的影响[J]. 水产科学, 2021, 40(6): 900-904.
LAI Xiaojian, PENG Shuai, ZHANG Zhe, LI Zhongqin. Testis Development Induced in Japanese Eel Anguilla japonica by 17α-Methyltestosterone Exposure. 水产科学, 2021, 40(6): 900-904.
[1]中国动物学会.中国动物科学研究[M].北京:中国林业出版社,1999:42-47. [2]Miura T, Miura C, Ohta T, et al. Estradiol-17β stimulates the renewal of spermatogonial stem cells in males[J].Biochemical and Biophysical Research Communications,1999,264(1):230-234. [3]张利红,张为民,林浩然.雄烯二酮和甲基睾酮诱导雄性鳗鲡性腺发育的作用[J].动物学研究,2001,22(2):105-109. [4]汪德海,龚小玲,鲍宝龙,等.长江口降海洄游鳗鲡的生长特性[J].上海海洋大学学报,2016,25(1):25-33. [5]Masuda Y, Imaizumi H, Oda K, et al. Artificial completion of the Japanese eel, Anguilla japonica, life cycle: challenge to mass production [J]. Bulletin of Fisheries Research Agency,2012(35):111-117. [6]Okamura A, Horie N, Mikawa N, et al. Recent advances in artificial production of glass eels for conservation of anguillid eel populations[J].Ecology of Freshwater Fish,2014,23(1):95-110. [7]曹伏君,叶宁,章俊杰.长鳍裸颊鲷精巢发育的组织学观察[J].水产科学,2017,36(1):54-59. [8]陈金涛,谢少林,王超,等.长臀鮠性腺发育及其组织结构观察[J].水产科学,2015,34(1):48-52. [9]方琼珊,翁幼竹,刘志刚,等.外源性促性腺激素诱导鳗鲡精子发生和成熟的作用机制[J].水产学报,2009,33(4):572-580. [10]张洁明,柳凌,郭峰,等.人工诱导鳗鲡性腺发育组织学研究[J].中国水产科学,2007,14(4):593-601. [11]Peñaranda D S, Pérez L, Gallego V, et al. Molecular and physiological study of the artificial maturation process in European eel males: from brain to testis[J].General and Comparative Endocrinology,2010,166(1):160-171. [12]Takeshi M, Kohei Y, Yoshitaka N, et al. Induction of spermatogenesis in male Japanese eel, Anguilla japonica, by a single injection of human chorionic gonadotropin(endocrinology)[J].Zoological Science,1991,8(1):63-73. [13]Lee S T L, Kime D E, Chao T M, et al. In vitro metabolism of testosterone by gonads of the grouper (Epinephelus tauvina) before and after sex inversion with 17α-methyltestosterone[J].General and Comparative Endocrinology,1995,99(1):41-49. [14]Miura T, Yamauchi K, Takahashi H, et al. Hormonal induction of all stages of spermatogenesis in vitro in the male Japanese eel (Anguilla japonica)[J].Proceedings of the National Academy of Sciences of the United States of America,1991,88(13):5774-5778. [15]Miura C, Miura T, Yamashita M, et al. Hormonal induction of all stages of spermatogenesis in germ-somatic cell coculture from immature Japanese eel testis[J].Development, Growth & Differentiation,1996,38(3):257-262. [16]Miura T, Miura C. Japanese eel: a model for analysis of spermatogenesis[J].Zoological Science,2001,18(8):1055-1063. [17]Miura T, Miura C I. Molecular control mechanisms of fish spermatogenesis[J].Fish Physiology and Biochemistry,2003,28(1/2/3/4):181-186. [18]Mayer I, Schmitz M, Borg B, et al.Seasonal endocrine changes in male and female Arctic charr (Salvelinus alpinus).I.Plasma levels of three androgens,17α-hydroxy-20β-dihydroprogesterone,and 17β-estradiol[J].Canadian Journal of Zoology,1992,70(1):37-42. [19]Amer M A, Miura T, Miura C, et al. Involvement of sex steroid hormones in the early stages of spermatogenesis in Japanese huchen (Hucho perryi)[J].Biology of Reproduction,2001,65(4):1057-1066. [20]李广丽,刘晓春,林浩然.17α-甲基睾酮对赤点石斑鱼性逆转的影响[J].水产学报,2006,30(2):145-150. [21]Song M, Gutzeit H O. Effect of 17-α-ethynylestradiol on germ cell proliferation in organ and primary culture of medaka (Oryzias latipes) testis[J].Development, Growth & Differentiation,2003,45(4):327-337. [22]Alsop D, Matsumoto J, Brown S, et al. Retinoid requirements in the reproduction of zebrafish[J].General and Comparative Endocrinology,2008,156(1):51-62. [23]Dufour S, Montero M, Belle N, et al. Differential distribution and response to experimental sexual maturation of two forms of brain gonadotropin-releasing hormone (GnRH) in the European eel, Anguilla anguilla[J].Fish Physiology and Biochemistry,1993,11(1/2/3/4/5/6):99-106.
2021, Vol. 40 
