Analysis of Drug-Resistance and Cross Resistance in Vitro of Vibrio alginolyticus Induced by Enrofloxacin
ZHANG Kai1,2, ZHANG Mingming1, XIE Xi1, WANG Rong1, HU Wenting1
1. Key Laboratory of Tropical Biological Resources, Ministry of Education, College of Life Sciences and Pharmacy, Hainan University, Haikou 570228, China; 2. Hainan Kangzhi Pharmaceutical Co., Ltd., Haikou 570311, China
Abstract：Enrofloxacin-sensitive Vibrio alginolyticus strains collected from the marine aquaculture area in Hainan province were induced by concentration-increasing method on the medium containing sub-inhibitory concentration of enrofloxacin in vitro to evaluate the occurrence of drug resistance. The minimal inhibitory concentration (MIC) of the induced bacterium to induced drugs and 6 non induced drugs was compared before and after drug resistance induction, and the genetic stability of the induced drug resistance strains was investigated on the media without drugs. The results showed that 24 enrofloxacin-sensitive strains were screened out of 30 strains of V. alginolyticus after 30 generations of induction in vitro. The sensitive strains were all resistant to enrofloxacin, and the MIC was 64—1024 times as large as before induction. The induced strains had severe cross-resistance to the non-induced quinolone drugs ciprofloxacin and norfloxacin, increase in resistance to tetracycline, a certain degree of enhancement to florfenicol and sulfadiazine, and no significant changes in sulfadiazine resistance. The cross resistance rates of 14 strains were over 50%, and that of HNVA21 was 83.3%. After 20 successive passages of drug-resistant progeny, the MIC value of 21 strains remained unchanged, and 3 strains were decreased by 1/2, with relatively stable drug resistance. Enrofloxacin led to induce drug resistance of V. alginolyticus with secondary inhibitory concentration, and cross-resistance to a variety of non-induced drugs, with good genetic stability of the resistant strains.
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2022, Vol. 41 
