Effects of Dietary Radix Glycyrrhizae Powder on Antioxidant Capacity of Asian Seabass Lates calcarifer under Low Salinity Stress
CHEN Xu, ZUO Tao, ZHOU Shengjie, YANG Rui, YU Gang, QIN Chuanxin, MA Zhenhua
1. Tropical Fisheries Research and Development Center/Sanya Tropical Fisheries Research Institute, South China Sea Fisheries Research Institute, ChineseAcademy of Fishery Sciences, Sanya 572018, China; 2. Key Laboratory of South China Sea Fishery Resource Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Abstract：In order to explore changes in the antioxidant capacity of Asian seabass Lates calcarifer under low-salt stress after feeding Radix Glycyrrhizae, Asian seabass with body weight of (13.89±2.50) g were reared in a 500 L fiberglass bucket and fed diets containing 0 (group 1), 10 (group 2), 30 (group 3) and 50 g/kg (group 4) of Radix Glycyrrhizae powder for 56 days at water temperature of 28.5 ℃ and salinity of 32.1. At the end of the culture, Asian seabass with body weight of (28.51±3.47) g was transferred to 100 L of freshwater plastics buckets filled with tap water for 24 hours, and then activities and contents of antioxidant-related enzymes were determined. The results showed that the activities of serum catalase and total antioxidant capacity in group 1 (control) were improved in varying degrees during the experiment. At the end of the low salinity stress, the activity of lysozyme was found to be increased with the increase in Radix Glycyrrhizae powder doses, the amount of malondialdehyde to be decreased; comparing to the treatment, and the activity of glutathione peroxidase and the amount of malondialdehyde to be decreased significantly (P<0.05). The activities of liver total superoxide dismutase, and peroxidase and total antioxidant capacity were decreased gradually with the increase in dietary Radix Glycyrrhizae powder content (P<0.05). Furthermore, the activities of peroxidase and total antioxidant capacity and the amount of malondialdehyde in each group after the experiment were significantly higher than ever before, while the activity of glutathione peroxidase was decreased. Except for group 2, the activity of Na+/K+-ATPase in gills in other groups after the experiment were significantly lower than ever before (P<0.05). In conclusion, the dietary Radix Glycyrrhizae powder can enhance the body vitality, anti-stress and anti environmental stress capabilities of juvenile Asian seabass.
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2023, Vol. 42 
