Development of Larva, Irreversible Starvation Point and Optimal Stocking Time of Phoxinus lagowskii Larva
REN Xingyue, LUO Xiaonian, DENG Shuping, DUAN Youjian, JIANG Yusheng, LI Jiao
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
Abstract：In order to study the developmental biological characteristics of Phoxinus lagowskii larva and optimal stocking time, microscopic observation of the P. lagowskii larval development, the larval starvation test and larva pond experiment were carried out. The observation of postembryonic development of larva showed that: under the condition of water temperature 16—18 ℃, at the beginning of their P. lagowskii length was (4.69±0.37) mm, yolk sac total length was (3.12±0.10) mm, 2 days post hatching (dph) the color of P. lagowskii eyespots became black, the digestive tract began versed at 5 dph, and the silver fish swim bladder began inflating one room at 6 dph, when the larval notochord began to bend and initial feeding was observed in the larvae at 7 dph, the yolk sac was completely exhausted at 9 dph, and the larvae showed second chamber of swim bladder at 12 dph, second bladder chamber aeration period at 21 dph, the larvae dorsal and anal fin emerged at 23 dph, at 27 dph larval ventral fins began to form, until at 35 dph, all fins were well developed in the larvae. The experiment of P. lagowskii irreversible starvation point results showed that: under 16—18 ℃, the P. lagowskii larvae first day of open mouth (at 7 dph) feeding rate was 36.36%. At 9 dph, the highest initial feeding rate was 85.71%, the irreversible starvation point was at 14 dph, the larvae of feeding rate was 40.91%. length (L) and age (t) von-bertalanffy growth formulaof juvenile fish before mouth open was L=8.0031[1-e-0.3139(t+2.8093)]. The optimal stocking time of P. lagowskii results showed that water temperature was 14.5—20.1 ℃, rotifer biomass keeps 8.30—18.58 mg/L, the highest survival rate and time of body mass growth appeared at 6 dph, the survival rate was (75.0±2.2)% and the larvae body mass reached (9.0±2.0) mg. Under the optimum total length of largest growth of timely for 8 dph, the minimum total length of the coefficient of variation was at 7.5 dph, minimum weight coefficient of variation of timely under the pond at was 8.5 dph. This experiment provides basic materials for post-embryonic development and optimal stocking time in P. lagowskii larva.
任星月, 骆小年, 邓舒萍, 段友健, 姜玉声, 李姣. 拉氏鱼岁仔稚鱼发育、饥饿不可逆点及适时下塘研究[J]. 水产科学, 2023, 42(4): 594-603.
REN Xingyue, LUO Xiaonian, DENG Shuping, DUAN Youjian, JIANG Yusheng, LI Jiao. Development of Larva, Irreversible Starvation Point and Optimal Stocking Time of Phoxinus lagowskii Larva. Fisheries Science, 2023, 42(4): 594-603.
[1]解玉浩.东北地区淡水鱼类[M].沈阳:辽宁科学技术出版社,2007:55-56. [2]董崇智.黑龙江水系鲤科鱼类名录[J].水产学杂志,2002,15(2):12-15. [3]骆小年,赵兴文,段友健.辽宁省淡水土著鱼类开发利用研究进展[J].大连海洋大学学报,2020,35(2):155-160. [4]牟振波,徐革锋,刘洋,等.洛氏鱼岁(Phoxinuslagowsrii)胚胎及仔鱼发育研究[J].东北农业大学学报,2014,45(7):98-103. [5]骆小年,李军,金广海,等.拉氏鱼岁人工繁殖试验[J].水产科学,2013,32(11):673-675. [6]骆小年,李军,杨培民,等.拉氏鱼岁池塘苗种培育试验[J].水产科学,2014,33(3):186-189. [7]刘艳辉,祖岫杰,刘铁钢,等.野生及养殖拉氏鱼岁营养成分比较分析[J].吉林农业大学学报,2017,39(1):82-88. [8]段国庆,江河,胡王,等.黄鳝仔鱼饥饿试验与不可逆点的确定[J].南方农业学报,2013,44(6):1036-1040. [9]强俊,李瑞伟,王辉.延迟投饵对奥尼罗非鱼仔鱼摄食、生长和存活的影响[J].淡水渔业,2008,38(5):60-64. [10]董艳珍,邓思红,肖文渊,等.延迟投饵对齐口裂腹鱼仔鱼摄食、存活和生长的影响[J].水产科技情报,2018,45(2):61-64. [11]乔志刚,常艳利,康青娟,等.延迟投饵对泥鳅仔鱼摄食、生长和存活的影响[J].水生态学杂志,2012,33(1):103-106. [12]骆小年,赵兴文,段友健.中国主要养殖经济鱼类人工催产药物使用进展[J].大连海洋大学学报,2020,35(1):10-18. [13]骆小年.中国主要经济鱼类鱼苗适时下塘研究进展[J].大连海洋大学学报,2021,36(1):1-9. [14]吴阿娜,王话翔,汤琳.两种溶解氧测定仪和碘量法测定地表水中溶解氧的比较和分析研究[J].环境科学与管理,2021,46(1):95-99. [15]章宗涉,黄祥飞.淡水浮游生物研究方法[M].北京:科学出版社,1991:358-382. [16]李艳华,危起伟,王成友.等.达氏鳇胚后发育的形态观察[J].中国水产科学,2013,20(3);585-591. [17]顾志敏,朱俊杰,贾永义,等.太湖翘嘴红鲌胚胎发育及胚后发育观察[J].中国水产科学,2008,15(2):204-214. [18]赖见生,杜军,赵刚,等.鲈鲤胚胎及胚后发育[J].西南农业学报,2014,27(3):1326-1331. [19]王磊,施文瑞,宋东蓥,等.草金鱼胚胎与胚后发育观察[J].水产科学,2018,37(6):742-748. [20]顾若波,徐钢春,闻海波,等.似刺鳊鮈的胚胎及胚后发育[J].中国水产科学,2008,15(3):414-424. [21]汪帆,杨瑞斌,樊启学.东方高原鳅的胚胎与胚后发育观察[J].华中农业大学学报,2017,36(6):89-98. [22]王丹,李文宽,闫有利,等.鸭绿江斑鳜胚胎及胚后发育观察[J].大连水产学院学报,2007,22(6):415-420. [23]马桂玉,李坚明,梁军能.三角鲤胚后发育研究[J].广东海洋大学学报,2011,31(4):37-42. [24]熊邦喜,庄平,庄振朋,等.长江鱼岁胚前和胚后发育的初步观察[J].华中农学院学报,1984,3(1):69-76. [25]王丹,赵晓临,王晓光,等.花鱼骨胚胎和仔鱼发育的初步研究[J].水产学杂志,2008,21(1):1-8. [26]徐晓津,王军,谢仰杰,等.大黄鱼消化系统胚后发育的组织学研究[J].大连水产学院学报,2010,25(2):107-112. [27]BLAXTER J H S, HEMPEL G. The influence of egg size on herring larvae (Clupea harengus L.)[J].ICES Journal of Marine Science,1963,28(2):211-240. [28]胡麟,吴天星.饥饿对鱼类生理生化的影响[J].水利渔业,2007,28(1):7-9. [29]WEN X B, CHEN L Q, KU Y M, et al. Effect of feeding and lack of food on the growth, gross biochemical and fatty acid composition of juvenile crab, Eriocheir sinensis[J].Aquaculture,2006,252(2/3/4):598-607. [30]王晓龙,温海深,张美昭,等.花鲈初孵仔鱼饥饿不可逆点的确定及摄食节律研究[J].中国海洋大学学报(自然科学版),2017,47(5):57-64. [31]菅玉霞,房慧,张少春,等.大泷六线鱼仔鱼饥饿试验及不可逆点的研究[J].海洋科学,2014,38(3):111-115. [32]STRÜSSMANN C A, TAKASHIMA F. Basic studies on the culture of pejerrey.II.Effects of temperature upon survival and histological changes of starved pejerreyOdontesthes bonariensis larvae[J].Nippon Suisan Gakkaishi,1989,55(2):247-254. [33]王敏.早期泥鳅的消化器官发育、摄食与生长的研究[J].水利渔业,2000,21(2):10-11. [34]李忠利,蒋天勇,巴家文,等.延迟首次投喂对乌江中华倒刺鲃仔鱼的补偿生长、消化酶活性及饵料日转化率的影响[J].淡水渔业,2020,50(2):72-78. [35]王吉桥,赵兴文.鱼类增养殖学[M].大连:大连理工大学出版社,2000:219-221. [36]王吉桥,王志余,张庆杰,等.鲤、鲢、鳙、草鱼苗最适下塘时间的研究[J].大连水产学院学报,1991,6(1):34-41. [37]李永函,孙大力,孙大江,等.延续池塘轮虫高峰期的试验[J].淡水渔业,1980,10(2):7-11.
2023, Vol. 42 
