Abstract：In order to understand the physiological characteristics of sperm of fat minnow Phoxinus lagowskii and improve its fertilization rate, we studied the effects of metal ion concentrations, temperature and pH on the sperm motility of fat minnow.The sperm fast moving time (FT) and life time (LT) were measured of 10 individuals of male fat minnow (body weight of 80.0—100.0 g and body length of 10.0—20.0 cm) under different ions [Na+, K+, Ca2+, glucose, and Tris buffer (C4H11NO3)], different temperatures [(8.0±1.0) ℃, (12.0±1.0) ℃, (16.0±1.0) ℃, (20.0±1.0) ℃, (24.0±1.0) ℃, and (30.0±1.0) ℃] and different pH (5.0, 6.0, 7.0, 8.0, and 9.0). The results showed that the sperm had the FT of (72.05±9.93) s and rising rate of 28.1%, the LT of (106.86±6.00) s with rising rate of 39.4%at Na+ concentration of 100 mmol/L. The sperm motility was the strongest at the K+ concentration of 75 mmol/L, and had the FT of (59.47±5.76) s with rising rate of 5.7%, and the LT of (252.11±218.64) s with rising rate of 228.9%, without no significant difference in the FT between 0 and 100 mmol/L glucose concentrations, but increase in LT with the increase in glucose concentration. In addition, Ca2+ and Tris buffer inhibited the sperm motility, with short FT and LT. The sperm motility was better with FT of (32.99±7.50) s and LT of(78.77±8.47) s at temperature of (20.0±1.0) ℃. The longest FT and LT was observed at pH 8.0, (45.42±6.56) s and (475.02±46.35) s. Without significant difference in the time of sperm rapid movement(745.67±273.08) s in compound solution B [V(Na+100 mmol/L)∶V(K+75 mmol/L)=1∶1],significantly higher life time than that in other conditions, with an increase rate of 968.6%. It was found that appropriate Na+, K+ and glucose concentration promoted the sperm motility of fat minnow, Ca2+ and that Tris buffer inhibited the sperm motility. The best sperm motility was observed in the fat minnow exosed to water temperature of (20.0±1.0) ℃ and pH range of 7.0—8.0. Compound solution B [V(Na+ 100 mmol/L)∶V(K+ 75 mmol/L)=1∶1] led to significant increase in sperm longevity.
吴晨, 骆小年, 段友健, 曾永聪, 缪云亮, 韩峰, 罗超. 不同离子浓度、温度、pH对拉氏鱼岁精子活力的影响[J]. 水产科学, 2022, 41(2): 210-217.
WU Chen, LUO Xiaonian, DUAN Youjian, ZENG Yongcong, MIAO Yunliang, HAN Feng, LUO Chao. Effects of Different Metal Concentrations, Temperature and pH on Sperm Motility of Fat Minnow Phoxinus lagowskii. 水产科学, 2022, 41(2): 210-217.
[1] 刘艳辉,陈伟强,刘铁钢,等.拉氏鱼岁的养殖现状及发展前景[J].水产科技情报,2015,42(5):255-257. [2] 骆小年,李军,金广海,等.拉氏鱼岁人工繁殖试验[J].水产科学,2013,32(11):673-675. [3] 骆小年,李军,杨培民,等.拉氏鱼岁池塘苗种培育试验[J].水产科学,2014,33(3):186-189. [4] 骆小年,赵兴文,段友健.辽宁省淡水土著鱼类开发利用研究进展[J].大连海洋大学学报,2020,35(2):155-160. [5] 骆小年,赵兴文,段友健.中国主要养殖经济鱼类人工催产药物使用进展[J].大连海洋大学学报,2020,35(1):10-18. [6] DUMORNÉ K, FIGUEROA E, COSSON J, et al. Protein phosphorylation and ions effects on salmonid sperm motility activation[J].Reviews in Aquaculture,2018,10(3):727-737. [7] LE M H, PHAM H Q. Sperm motilities in waigieu seaperch, Psammoperca waigiensis:effects of various dilutions, pH, temperature, osmolality, and cations[J].Journal of the World Aquaculture Society,2017,48(3):435-443. [8] 刘鉴毅,甘芳,危起伟,等.几种不同浓度的离子及单糖对中华鲟精子活力的影响[J].水生生物学报,2007,31(6):849-854. [9] 周定刚,温安祥.黄鳝精子活力检测和精子入卵早期过程观察[J].水产学报,2003,27(5):398-402. [10] 苏天凤,艾红.鱼类精子活力及其超低温保存研究综述[J].上海水产大学学报,2004,13(4):343-347. [11] MORISAWA M, MORISAWA S. Acquisition and initiation of sperm motility[M]//GAGNON C. Controls of Sperm Motility.Los Angeles:CRC Press,2020:137-151. [12] BILLARD R, COSSON J, CRIM L W. Motility of fresh and aged halibut sperm[J].Aquatic Living Resources,1993,6(1):67-75. [13] 张涛,李胜忠,牛建功,等.Na+、K+、葡萄糖及甘油对高体雅罗鱼精子活力的影响[J].南方农业学报,2017,48(4):734-738. [14] 阮国良,冯家斌,杨代勤.渗透压、pH和温度对泥鳅精子活力及受精率的影响[J].湖北农学院学报,2004,24(1):22-25. [15] 赵振山,曹克驹,杨立军.乌鳢精子生理生态特性的研究Ⅰ:不同pH溶液对精子活力的影响[J].水利渔业,1995,16(3):11-12. [16] 韩峰,骆小年,段友健,等.Na+、K+、Ca2+、葡萄糖、pH、温度对北方须鳅精子活力的影响[J].大连海洋大学学报,2021,36(3):462-469. [17] 严安生,王其和,李诗模.渗透压和钾对鲤、团头鲂精子活力的影响[J].淡水渔业,1993,23(3):19-21. [18] 邓岳松,林浩然.鱼类精子活力研究进展[J].生命科学研究,1999,3(4):271-278. [19] 魏开金,王汉平,林加敬,等.氯化钠浓度对鲥鱼精子活力影响的初步观察[J].淡水渔业,1996,26(4):9-10. [20] 苏德学,严安生,田永胜,等.阳离子、葡萄糖及渗透压对丁鱼岁精子活力的影响[J].水利渔业,2004,25(1):7-8. [21] 王位莹,李胜忠,张俊杰,等.江鳕精子在不同激活液中的活力测定[J].贵州农业科学,2015,43(10):144-146. [22] 王永明,陈瑜,胡雨,等.宽体沙鳅精子超微结构及Na+、K+、Ca2+对其精子活力的影响[J].四川动物,2016,35(1):38-43. [23] 吴清毅,孙翰昌,李云瑶.不同浓度的K+、Ca2+和葡萄糖对乌原鲤精子活力的影响[J].水产科学,2011,30(4):202-205. [24] 丁辰龙,王宣朋,李康,等.NaCl、KCl和葡萄糖对大鳞鲃精子活力的影响[J].天津师范大学学报(自然科学版),2018,38(6):32-35. [25] 王芳,岳兴建,谢碧文,等.pH、葡萄糖以及金属离子对翘嘴红鮊精子活力的影响[J].安徽农业科学,2011,39(27):16812-16814. [26] 闫家强,魏平,姜建湖,等.环境因子及超低温冻存对黄姑鱼精子活力的影响[J].生态科学,2010,29(4):339-344. [27] 徐万土,戴海平,程顺,等.细鳞斜颌鲴精子活力观察[J].浙江海洋学院学报(自然科学版),2013,32(4):309-314. [28] ALAVI S M H, COSSON J, KARAMI M, et al. Spermatozoa motility in the Persian sturgeon, Acipenser persicus:effects of pH, dilution rate, ions and osmolality[J].Reproduction (Cambridge, England),2004,128(6):819-828. [29] 刘新峰,郝志明,孙忠媛,等.Tris-葡萄糖稀释液中添加重酒石酸去甲肾上腺素、丙酸睾酮对低温保存犬精子活率的影响[J].黑龙江畜牧兽医,2009(3):115-116. [30] 刘雄,王昭明,金国善,等.虹鳟养殖技术[M].北京:农业出版社,1990. [31] 严安生,宋贵文,闫拥军.鲤、团头鲂精子生理生态特性的研究—Ⅲ:单糖和渗透压对精子活力的影响[J].淡水渔业,1995,25(2):3-5. [32] KRUGER J C D W, SMIT G L, VAN VUREN J H J, et al. Some chemical and physical characteristics of the semen of Cyprinus carpio L.and Oreochromis mossambicus (Peters)[J].Journal of Fish Biology,1984,24(3):263-272. [33] 苏德学,严安生,田永胜,等.钠、钾、钙和葡萄糖对白斑狗鱼精子活力的影响[J].动物学杂志,2004,39(1):16-20. [34] 丁淑荃,李飞,万全,等.不同浓度葡萄糖、NaCl和KCl对蛇鮈精子活力的影响[J].安徽农业大学学报,2012,39(6):881-884. [35] 罗明坤,赵岩,THAMMARATSUNTORN J,等.pH、盐度及K+、Ca2+和葡萄糖对萨罗罗非鱼精子活力的影响[J].南方农业学报,2014,45(11):2076-2080. [36] 梁伟峰,张海发,王云新,等.几种因子对鞍带石斑鱼精子活力的影响[J].广东海洋大学学报,2009,29(3):29-32. [37] 罗芬,黄伟卿,阮少江,等.温度对海捕野生大黄鱼精子活力的影响[J].水产科学,2017,36(6):814-817. [38] 顾正选,丁诗华.pH值及不同百分浓度NaCl溶液对齐口裂腹鱼精子活力的影响[J].西南大学学报(自然科学版),2017,39(7):72-76. [39] 杨彩根,宋学宏,王永玲.pH及不同浓度NaCl溶液对黄颡鱼精子活力的影响[J].水利渔业,2003,24(3):10-11. [40] 李加儿,区又君,江世贵.环境因子变化对平鲷精子活力的影响[J].动物学杂志,1996,31(3):6-9. [41] 谢刚,叶星,苏植蓬,等.鳗鲡精子的主要生物学特性[J].上海水产大学学报,1999,8(1):81-84.
2022, Vol. 41 
