摘要为探讨重金属对贝类早期生活史阶段生理生化指标的影响及可能的毒性机制，以太平洋牡蛎的胚胎和幼虫为材料，研究重金属汞对其早期发育阶段3种与免疫防御等功能相关酶活力的影响。试验结果显示，3μg/L、30μg/L和300μg/L的汞暴露2 h （桑葚胚期）、5 h （原肠期）、8 h （担轮幼虫期）和20 h （D形面盘幼虫期），胚胎和幼虫的酸性磷酸酶和碱性磷酸酶的活力水平均显著低于对照组（ P＜0．01）；3μg/L和30μg/L汞暴露后过氧化物酶的活力也受到显著抑制（ P＜0．01），而300μg/L的汞暴露后的过氧化物酶活力较对照组显著升高（P＜0．01）。对照组这3种酶的活力随发育期而变化，其活力水平值均在桑葚期有一个升高，原肠胚期和担轮幼虫期较低，而D形面盘幼虫期又有所升高。本研究结果表明，重金属对酸性磷酸酶、碱性磷酸酶及过氧化物酶活力的影响可能是其导致双壳贝类胚胎和幼虫毒性的原因之一。
Abstract：The effects of heavy metal Hg on three defense related enzyme activities were investigated in embryos and larvae of Pacific oyster Crassostrea giga to elucidate the effects of heavy metals on physiological and biochemical indexes and toxic mechanism during early developmental stages of bivalves . The results showed that the acid phosphatase (ACP) and alkaline phosphatase (ALP) activities were found to be reduced significantly (P<0 .01) in embryos and larvae exposed to Hg concentrations of 3 μg/L ,30μg/L and 300 μg/L for 2 h (morula stage) ,5 h (gastrula stage) ,8 h (trochophore larval stage) ,and 20 h (D-shaped veliger larval stage) compared with those in control group .The peroxidase (POD) activities of embryos and larvae were also found to be inhibited significantly (P<0 .05) in the groups of 3μg/L and 30μg/L compared with the control group ,while the POD activity were increased in 30 μg/L group (P<0 .01) .In control group ,three enzyme activities were changed with the development of Pacific oyster , with increases in activity in morula stag ,then decrease ,the minimum in gastrula and trochophore stages and increase in veliger larvae .The findings indicate that the toxic effects of metal on embryos and larvae of bivalves might be involved in the changes in ACP ,ALP and POD activities .
收稿日期: 2014-01-25
引用本文:
梁建光,白雪峰,巩淇,付正阳,孙蕾,王宜艳. 汞对太平洋牡蛎早期发育阶段三种防御相关酶活力的影响[J]. 水产科学, 2014, (1): 35-39.
LIANG Jian-guang,BAI Xue-feng,GONG Qi,FU Zheng-yang,SUN Lei,WANG Yi-yan. Effects of Hg on Three Defense Related Enzyme Activities in Early Developmental Stages of Pacific Oyster Crassostrea giga. Fisheries Science, 2014, (1): 35-39.
