Effects of Four Inonotus obliquus Polysaccharides on Growth Performance, Serum Biochemical Indices,Non-specific Immunity and Activities of Related Antioxidant Enzymes in Pacific White Leg Shrimp Litopenaeus vannamei
LIU Xiao-ling,XU Xiang-qun,HUANG Yan-hua,CAO Jun-ming,WANG Guo-xia,MO Wen-yan,WU Chun-yu,FU Jing-jing
Abstract：A total of 450 individuals of Pacific white leg shrimp Litopenaeus vannamei with initial body weight of (0 .52 ± 0 .01) g was randomly allocated into 5 groups and fed the basal diet (control group) and the basal diet supplemented with four different Inonotus obliquus polysaccharides (IPO1 ,IPO2 ,IPO3and IPO4) at a rate of 1000 mg/kg diet for 28 days to evaluate the effects of the Inonotus obliquus polysaccharides on grow th performance ,serum biochemical indices ,non-specific immunity and activities of related antioxidant enzymes .It was found that there were significantly higher weight gain rate (WGR) , specific growth rate (SGR) and significantly lower feed coefficient (FC) in IPO2 ,IPO3 ,and IPO4 than those in control group (P< 0 .05) .The shrimp in IPO4 had significantly higher food intake (FI) ,and survival rate (SR) than those in control group ( P<0 .05) .No significant differences in condition factors (CF) and hepato-somatic indices (HSI) were found among all groups (P>0 .05) .There were significantly higher crude lipid contents in the shrimp in IPO1 ,IPO2 ,IPO3 ,and IPO4 than that in control group (P<0 .05) .However ,there were significantly lower in ash contents in the shrimp in IPO 2 ,IPO3 ,and IPO4 , and significantly lower in serum uric acid (UA) contents in the shrimp in IPO2 ,and IPO4 (P<0 .05) . Serum lysozyme (LSZ) and total antioxidant capacity (T-AOC) were shown to be increased significantly in IPO3 and IPO4 (P<0 .05) ,and the serum alkaline phosphatase (AKP) activity be significantly higher in the shrimp in IPO4 than that in the others ( P<0 .05) .Dietary Inonotus obliquus polysaccharides led to increase in serum phenol oxidase (PO ) ,superoxide dismutase (SOD ) ,anti-superoxide anion (anti-O2-) activities ,without significant difference ( P>0 .05) .Serum malondialdehyde (MDA ) content was found significantly lower in IPO4 than that in control group (P< 0 .05) .In conclusion ,four dietary Inonotus obliquus polysaccharides result in improvement of grow th performance and increase in activities of serum lysozyme ,total antioxidant capacity ,and alkaline phosphatase ,and in decrease in serum malondialdehyde content in the shrimp ,especially the Inonotus obliquus polysaccharide .
收稿日期: 2014-04-25
引用本文:
刘小玲,徐向群,黄燕华,曹俊明,王国霞,莫文艳,吴春玉,付晶晶. 桦褐孔菌多糖对凡纳滨对虾生长和血清免疫相关酶活性的影响[J]. 水产科学, 2014, (4): 201-207.
LIU Xiao-ling,XU Xiang-qun,HUANG Yan-hua,CAO Jun-ming,WANG Guo-xia,MO Wen-yan,WU Chun-yu,FU Jing-jing. Effects of Four Inonotus obliquus Polysaccharides on Growth Performance, Serum Biochemical Indices,Non-specific Immunity and Activities of Related Antioxidant Enzymes in Pacific White Leg Shrimp Litopenaeus vannamei. Fisheries Science, 2014, (4): 201-207.
