Cloning and Derived Antibacterial Peptides of Histone H2A Gene in Black Carp Mylopharyngodon piceus,Grass Carp Ctenopharyngodon idellus,Silver Carp Hy pophthalmichthys molitrix and Bighead Carp Aristichthys nobilis
Abstract：Antimicrobial peptide derived from Histone H2A ,one of the chromosome structure protein ,is a cationic antimicrobial peptide showing a potential to substitute antibiotics .The characterization of histone H2A and the derivative antibacterial peptides are important for understanding of the mechanism and antibacterial difference among peptides derived from different H 2A genes in different fish .In this study , RT‐PCR was used to amply the H2A cDNA with the total RNA as the template in black carp Mylopharyngodon piceus , grass carp Ctenopharyngodon idellus , silver carp Hypophthalmichthys molitrix and bighead carp A ristichthys nobilis .The homologous H2A gene sequences were selected in the BLAST in GenBank ,and aligned in software Mega5 . The universal primers were designed in software Primer5 to amplify H2A genes collected and linked with a T‐vector ,and transferred into the competent cells for cloning .The H2A gene sequences with the length of 364 bp in the four fish species were cloned , and the deduced proteins were found to be unstable ,with the PI value of 10 .48—11 .02 and the total average hydrophilicity of 0 .179—0 .264 by online analysis . Both sequence homology analysis and the constructed phylogenetic tree revealed that the deduced H2A proteins showed the high homology (over 85% ) in four fish species with those in Sinocyclocheilus angustiporus and zebrafish .Histone H2A‐derived antibacterial peptide located in N terminal region in the four fish species showed the high homology with Hipposin in Atlantic halibut H ip poglossus hip poglossus .In addition ,there were glutamic acid residues in all amino acid sequences . It was confirmed that the antibacterial peptides derived from H 2A include Hipposin ,Parasin Ⅰ ,and Buforin ,belonging to cationic peptides with αspiral structure .
收稿日期: 2014-05-25
引用本文:
武向敏,赵燕静,孔祥会,江红霞,李莉,聂国兴,李学军. 青鱼、草鱼、鲢鱼和鳙鱼组蛋白 H2A N-端基因克隆及其衍生抗菌肽[J]. , 2014, (5): 311-316.
WU Xiang-min,ZHAO Yan-jing,KONG Xiang-hui,JIANG Hong-xia,LI Li,NIE Guo-xing,LI Xue-jun. Cloning and Derived Antibacterial Peptides of Histone H2A Gene in Black Carp Mylopharyngodon piceus,Grass Carp Ctenopharyngodon idellus,Silver Carp Hy pophthalmichthys molitrix and Bighead Carp Aristichthys nobilis. , 2014, (5): 311-316.
