摘要24～25℃，研究了间氨基苯甲酸乙酯甲磺酸盐对厚颌鲂幼鱼的麻醉效果。根据厚颌鲂幼鱼在麻醉和复苏过程的行为特征，将其麻醉过程分为5期，复苏过程分为4期。试验结果表明，厚颌鲂幼鱼的入麻时间随麻醉剂质量浓度的升高不断缩短（F＝430．263，P＜0．01），在该试验条件下，间氨基苯甲酸乙酯甲磺酸盐麻醉厚颌鲂幼鱼的有效质量浓度为70～120 mg／L ；麻醉过程中厚颌鲂幼鱼的呼吸频率先急剧升高（应激期～镇静期），然后缓慢下降（镇静期～深度麻醉期），直至过度麻醉停止呼吸（死亡期）；深度麻醉状态下，试验鱼在空气中的暴露时间与复苏时间呈正相关（F＝145．416，P＜0．01），除短时间暴露的2个试验组（3 min和4 min）复苏时间差异不显著（P＞0．05）外，其余各组差异均极显著（P＜0．01）；暴露时间在5 min以内，复苏率100％，超过5 min ，只有部分试验鱼可以复苏，当暴露时间达到12 m in时，厚颌鲂幼鱼全部死亡。
Abstract：The anaesthetic effect of MS‐222 on Megalobrama pellegrini juveniles was studied in water tanks in a laboratory at water temperature of 24—25 ℃ .In terms of the behavior characteristic of the fish , the anaesthetia was divided into five stages ,and the recovery process was divided into four stages .The fish was found to be anaesthetized more rapidly with increase in the concentration of MS‐222 (F=430 .263 ,P< 0 .01) ,with effective dose of 70—120 mg/L for anaesthetia . The fish showed rapid increase in respiratory frequency during the anaesthetic stage 1 and stage 2 first ,and then decline slowly from stage 2 to stage 4 ,until the fish stopped opercula moving in the stage of 5 due to excessive anaesthetia .There was a positive correlation between exposure time to air and recovery time for deeply anaesthetized fish (F=145 .416 ,P < 0 .01) ,significant difference(P<0 .01) among different exposure time groups except the two shorter exposure groups(3 min and 4 min) .The fish had recovery rate of 100% when the fish was exposed to MS‐222 within 5 min ,and only a few fish was recovered over 5 min .The fish came to death when the fish was exposed to MS‐222 for 12 min .
收稿日期: 2014-06-25
引用本文:
曲焕韬,刘勇,郭文韬,肖衎,管敏,李莎,鲁雪报. 间氨基苯甲酸乙酯甲磺酸盐对厚颌鲂幼鱼麻醉效果的研究[J]. 水产科学, 2014, (6): 374-378.
QU Huan-tao,LIU Yong,GUO Wen-tao,XIAO Kan,GUAN Min,LI Sha,LU Xue-bao. The Anaesthetic Effect of MS-222 on Megalobrama pellegrini Juveniles. Fisheries Science, 2014, (6): 374-378.
