Abstract:Aeromonas hydrophila is one of the main pathogen of freshwater fish bacterial septicemia .The bacterium was isolated and identified from dead gold fish and drug sensitive tests were performed in order to provide references for the bacterial disease prevention and control in ornamental fish .A dominant bacteria strain was isolated from hepatopancreas of dead Carassius auratus and identified by artificial infection experiment ,cultural characteristics ,physical and chemical characters ,and 16S rRNA sequence analysis .The results showed that the strain was A .hydrophila .Homology of 16S rRNA of the isolated strain and other several A .hydrophila was more than 99 .3% .Drug sensitive test revealed that the isolated strain was highly sensitive to 21 kinds of drugs , including tetracyclines ,quinolones , sulfonamides , cefuroxime ,ceftazidimeand cefepime ,and resistant to penicillin G ,ampicillin ,amoxicillin ,cefalexin , lincomycin and medemycin . Results in this study showed that many antibiotics (such as doxycycline , midecamycin ,co‐trimoxazole ,sulfamethoxazole ,azithromycin ,enrofloxacin ,and norfloxaci) can be used to control and prevent bacterial disease caused by A .hydrophila in gold fish .
收稿日期: 2014-06-25
引用本文:
周毅,张培培,徐晔,曹洁,孟学平,段宏安. 金鱼嗜水气单胞菌的分离鉴定及药敏试验[J]. 水产科学, 2014, (6): 379-384.
ZHOU Yi,ZHANG Pei-pei,XU Ye,CAO Jie,MENG Xue-ping,DUAN Hong-an. Identification and Drug Sensitivity Test of Pathogenic Aeromonas hydrophila from Goldfish Carassius auratus. Fisheries Science, 2014, (6): 379-384.