Characterization of Bacterial Communities Associated with the Intestinal Tracts and Culture Environment of Sea Cucumber (Apostichopus japonicus) in Different Culture Seasons
LI Jian-guang,XU Yong-ping,LI Xiao-yu,WANG Li-li,BAO Peng-yun,PU Hong-yu,CHE Jian,WANG Xi-tao
Abstract：The aim of the study was to use a combination of denaturing gradient gel electrophoresis tech-nology to evaluate the bacterial communities of artificial diet-fed sea cucumber (Apostichopus japonicus) adults in different culture seasons .Overall ,the results showed that bacterial communities associated with sea cucumbers were considerable stable in different culture seasons ,little differences in the bacterial as-semblages .Phylogenetic analysis demonstrated that the most frequently observed species were γ-Pro-teobacteria and α-Proteobacteria ,additional species were β-Proteobacteria ,ε-Proteobacteria ,Spirochaet-ales ,Bacteroidetes ,Cytophagales and Chlamydiales groups .The dominant bacterial community composi-tion including V ibrio sp ., Roseobacter sp ., Photobacterium sp . in intestine samples ;V ibrio sp ., Ro-seobacter sp .,Cytophage sp .was the dominant group in the seawater samples ;while Vibrio sp .,Micro-coccus sp .,Pseudoalteromonas sp .,Photobacterium sp .,Chloroflexi sp .,Shewnella sp .,Pantoea sp ., X anthomonas sp .and ,A grobacterium sp .were the dominant group in the sediment samples .These find-ings provide the sequence foundation for the development of molecular assays that target sea cucumber bac-teria and probiotics .
收稿日期: 2014-09-25
引用本文:
李建光,徐永平,李晓宇,王丽丽,包鹏云,蒲红宇,车鉴,王熙涛. 不同养殖季节仿刺参肠道与养殖环境中菌群结构的特点[J]. 水产科学, 2014, (9): 562-568.
LI Jian-guang,XU Yong-ping,LI Xiao-yu,WANG Li-li,BAO Peng-yun,PU Hong-yu,CHE Jian,WANG Xi-tao. Characterization of Bacterial Communities Associated with the Intestinal Tracts and Culture Environment of Sea Cucumber (Apostichopus japonicus) in Different Culture Seasons. Fisheries Science, 2014, (9): 562-568.
