Abstract:The experiment was conducted to study the effects of homology Lactobacillus preparation with different strains in diets on immune ,antioxidant and resistance against Aeromonas hydrophila .The tilapia hybrid (Oreochromis niloticus × O . aureu) with initial body weighg of 3 .49 g were fed the basal diet in control group ,and fed the basal diet containing L .acidophilus ,L .plantarum ,L .pentosus ,L .rhamno-sus and Enterococcus f aecalis in the other five groups for 8 weeks .The number of live lactic acid bacteria in each L actobacillus used in the experiment was 1 .30 × 103 ,5 .10 × 108 ,2 .97 × 108 ,3 .03 × 108 ,2 .30 × 108 and 3 .47 × 108 cfu /g ,respectively .At the end of the feeding test ,the fish were infected with Aeromonas hydrophila for 72 hours .The serum acid phosphatase activity was increased in varying degrees ,the maxi-mum in L .p lantarum group ,L .pentosus group ,L .rhamnosus group and E . f aecalis group in L .pento-sus group the highest was ,significantly higher than that in L .acidophilus group .The serum antisuperox-ide anion free radical activities were increased significantly ,higher in L .rhamnosus and E .f aecalis groups compared with the others .The activity of serum lysozyme was significantly higher in L .rhamnosus group than in the control group .During injection of A .hydrophilain 12 h ,the survival rate was higher in Lacto-bacillus groups than in the control ,no significant difference .In 24 and 48 hours ,survival rate were sig-nificantly higher in the L . rhamnosus and E . f aecalis groups than control group .In 72 hours ,survival rate in the L .rhamnosus group was significantly higher than control group .The results suggested that the supplementation of L actobacillus into the practical diet enhanced anti-oxidation ,and immune capacity and improved the resistance against A .hydrophila in tilapia .
收稿日期: 2014-10-25
引用本文:
黄燕华,周晓波,王国霞,邝哲师,赵红霞,莫文艳,曹俊明. 5种乳酸菌对奥尼罗非鱼免疫和抗病力的影响[J]. 水产科学, 2014, (10): 601-605.
HUANG Yan-hua,ZHOU Xiao-bo,WANG Guo-xia,KUANG Zhe-shi,ZHAO Hongxia,MO Wenyan,CAO Junming. Effects of Lactobacillus on Immunity and Disease Resistance of Tilapia (Oreochromis niloticus × O . aureu). Fisheries Science, 2014, (10): 601-605.