Abstract：An ivitro experiment was conducted to evaluate the effects of glutamine (0 ,0 .5 ,1 ,2 mmol/L) on immune responses of kidney leukocytes in grouper (Epinephelus malabaricus) .After the leukocytes were cultured primarily in 2 ,6 ,12 ,24 h ,the phagocytosis and respiratory burst activity of leukocytes were determined ,respectively .Phagocytosis and respiratory burst activity of leukocytes were significantly enhanced with glutamine supplemented into the culture medium ,and increased further with the increasing culture time .The peak was occurred in 1 mmol/L glutamine group 12 h after incubation .The proliferation of leukocytes was determined 12 and 24 h after incubation in medium supplemented with glutamine .After 12 h incubation ,the proliferation effects in the groups supplemented with glutamine were significantly im-proved compared with the basal group ,and no significant differences were observed in groups supplemen-ted with glutamine ,as well as the highest proliferation activity was found in 1 mmol/L glutamine group . And the proliferation activity was decreased 24 h after incubation except 2 mmol/L glutamine group .The trend of killing ability against E .tarda in the leukocytes was similar with the phagocytosis .The killing a-bility of leukocytes incubated in medium supplemented in 1 and 2 mmol/L glutamine were significantly higher than that in basal group (P<0 .05) ,and no significant difference was observed in 0 .5 mmol/L glu-tamine group and the basal group (P>0 .05) .The findings indicate that glutamine is the effective immu-nomodulatory in grouper leukocytes ,and the optimum glutamine supplemental level 1 mmol/L at the incu-bation time of 12 h .
收稿日期: 2014-10-25
引用本文:
程镇燕,李建,雷五长,于宏,王庆奎,郭永军,乔秀亭. 谷氨酰胺对点带石斑鱼免疫细胞免疫力的影响[J]. 水产科学, 2014, (10): 606-610.
CHENG Zhen-yan,LI Jian,LEI Wu-chang,YU Hong,WANG Qing-kui,GUO Yong-jun,QIAO Xiu-ting. Effects of Glutamine on Immune Responses of Immunocytes in Grouper Epinephelus malabaricus. Fisheries Science, 2014, (10): 606-610.
