Optimization and Analysis of Two-dimensional Electrophoresis of Hepatopancreas Proteins in Chinese Shrimp (Fenneropenaeus chinensis) under Crowding Stress
Abstract：The two-dimensional electrophoresis (2-DE) was optimized and differentially expressed proteins were investigated in hepatopancreas of Chinese shrimp (Fenneropenaeus Chinensis) under crowded condi-tion .The results showed that the optimal two-dimensional electrophoresis were observed under the condi-tions of addition of TBP (tributyl phosphate) ,strips of 17 cm ,and pH 4-7 IPG (Immobilized pH gradi-ent) ,loading the sample of 120 μg and setting the voltage and power of isoelectric focusing as 65 000 Vhr . The relative 2-DE maps showed that approximate 600 spots was detected in each gel ,and a total of 11 dif-ferentially expressed protein spots were found ,including 8 significantly up-regulated protein spots (P <0 .05) and 3 significantly down-regulated protein spots (P < 0 .05) .The findings indicated that proteins in the shrimp hepatopancreas were differently expressed under crowding stress .
收稿日期: 2014-10-25
引用本文:
张帅,傅玲琳,王彦波. 拥挤条件下中国明对虾肝胰脏蛋白质双向电泳条件优化与分析[J]. 水产科学, 2014, (10): 621-625.
ZHANG Shuai,FU Ling-lin,WANG Yan-bo. Optimization and Analysis of Two-dimensional Electrophoresis of Hepatopancreas Proteins in Chinese Shrimp (Fenneropenaeus chinensis) under Crowding Stress. Fisheries Science, 2014, (10): 621-625.
