摘要随机采取不同日龄健康的牙鲆仔鱼，用电子目镜和天平测定其全长（L）、体质量（m），运用整体匀浆法测定鱼体中核酸和总蛋白浓度、RNA／DNA （a）比值和总蛋白／DNA比值（b），研究了牙鲆仔鱼的生长，DNA、RNA和总蛋白的变化规律，探讨了核酸、总蛋白和生长的关系。试验结果表明，牙鲆仔鱼体质量、全长与RNA／DNA比值均呈线性负相关，关系式分别为 m＝-19．85 a＋82．79（r2＝0．89， P＜0．01），L＝-7．41 a＋33．53（r2＝0．85，P＜0．05）。上述关系式说明，牙鲆仔鱼时期的生长以细胞增殖为主，大量消耗前期合成的蛋白质。牙鲆仔鱼体质量、全长与总蛋白／DNA 比值也呈线性负相关，关系式分别为 m＝-0．61 b ＋34．54（r2＝0．93， P ＜0．01），L＝-0．22 b ＋15．33（r2＝0．86， P ＜0．01）。随着牙鲆仔鱼养殖日龄的增加，总蛋白／DNA比值下降的幅度大于RNA／DNA比值。核酸指标能从细胞水平上分析仔鱼的生长。
Abstract：Total length (L) and body weight (m) ,concentrations of nucleic acid ,and protein ,RNA/DNA ratio (a) ,and protein/DNA ratio (b) were measured in larvae of Japanese flounder Paralichthys oliv aceus by whole homogenate method and by electron microscope to evaluate the relationship between nucleic indi-cators and growth in larval Japanese flounder .It was found that body weight and total length were nega-tively correlated with RNA/DNA ratio ,with an equation as m= -19 .85 a + 82 .79 (r2 = 0 .89 , P<0 .01) ,and as L= -7 .41 a + 33 .53(r2 = 0 .85 , P<0 .05) ,indicating that the grow th of Japanese floun-der larvae was primarily involved in hyperplasia and in exhaustion of protein synthesized during the early developmental stage .Body weight and total length were also negatively correlated with protein :DNA rati-o ,with an equation m= -0 .61b + 34 .54 (r2 = 0 .93 ,P<0 .01) and L= -0 .22b + 15 .33 (r2 = 0 .86 ,P<0 .01) .There was larger decrease scope in the protein/DNA ration than that in the RNA/DNA ratio as the larvae elapse in culture .The findings suggest that nucleic indicators be used to evaluate growth rates of larval Japanese flounder on a cellular level .
收稿日期: 2014-10-25
引用本文:
佟雪红,王欢莉,葛宝明,杨小兰,张振铭. 牙鲆仔鱼核酸与生长关系的研究[J]. 水产科学, 2014, (10): 639-642.
TONG Xue-hong,WANG Huan-li,GE Bao-ming,YANG Xiao-lan,ZHANG Zhen-ming. Relationship between Nucleic Indicators and Growth in Larvae of Japanese Flounder Paralichthys olivaceus. Fisheries Science, 2014, (10): 639-642.
