Effect of Licorice on Antioxidant Enzyme Activity in Hepatopancreas and Micronucleus Number in Red Blood Cell of Crucian Carp Carassius auratus exposed to Cu2+ Stress
WANG Shi-feng,XU Ping,GULIMIRE Anwaier,REMILA Musha,WU Yong-jun
Abstract：Crucian carp (Carassius auratus) was bathed in tapwater (control group) ,water containing cop‐per and water supplemented with licorice for 7 days to evaluate detoxification function of licorice and re‐sponse of crucian carp to Cu2+ stress w ere studied via antioxidant enzyme activity ,Cu2+ content and micro‐nucleus levels in red cells . Results showed that there were significantly higher superoxide dismutase (SOD) ,and peroxidase (POD) activities and malonaldehyde (MDA) content in the fish bathed in the wa‐ter containing copper than those in the control group when the fish were bathed in the Cu2+ containing wa‐ter for 2 ,3 and 4 days .The activities of SOD and POD were increased significantly as Cu2+ exposure elasped ,but MDA content gradually decreased .The fish bathed in the licorice containing water had lower SOD ,and POD activities and MDA content in hepatopancreas than the fish in the control group .There was Cu2+ content of 13 .08 mg/kg and 14 .96 mg/kg in hepatopancreas of the fish bathed in the Cu2+ con‐taining water for 4 and 7 days ,significantly higher than those in the fish bathed in the licorice containing water (10 .83 mg/kg and 11 .64 mg/kg) .There was significantly lower micronucleus rate in the fish bathed in the licorice containing water(3 .17% ) than that in the fish bathed in the Cu2+ containing water (4 .17% ) for 7days ,indicating that licorice has a certain detoxification function .
收稿日期: 2014-11-25
引用本文:
王世锋,徐萍,古丽米热·安外尔,热米拉·木沙,吴永俊. 甘草对铜胁迫下鲫鱼肝胰脏的抗氧化酶活性及血液红细胞中微核数量的影响[J]. 水产科学, 2014, (11): 713-717.
WANG Shi-feng,XU Ping,GULIMIRE Anwaier,REMILA Musha,WU Yong-jun. Effect of Licorice on Antioxidant Enzyme Activity in Hepatopancreas and Micronucleus Number in Red Blood Cell of Crucian Carp Carassius auratus exposed to Cu2+ Stress. Fisheries Science, 2014, (11): 713-717.
