Abstract：Identification was carried out in 128 strains of Streptococcus isolated from tilapia in southern China (Guangdong ,Guangxi and Hainan provinces ) from 2010 to 2011 by Bio‐MerieuxVITEK and 16S rRNA sequence analysis and 7 housekeeping genes were seguenced in 104 strains of S .agalactiae by Mul‐tilocus Sequence Typing (MLST ) technique to evaluate the genetic diversity of S . agalactiae .Results were analyzed and compared with sequence information in PubMLST databases to determine a strain allelic profile and assigned a sequence type (ST ) .It was found that total 104 strains were within 3 clone se‐quences :ST‐7 and 2 types of unidentified NST‐1 and NST‐2 ,NST‐1 having the maximal proportion of 88 .5% (92/104) .It was revealed that there were multiple clonal sequences of S .agalactiae isolate from tilapia in southern China with high pathogenetic and prevalent .It is suggested that S . agalactiae isolate from tilapia be intense surveillance efforts .The findings indicate that MLST technique is an ideal tool to reveal genetic evolution profile of S .agalactiae isolated from tilapia in southern China .
收稿日期: 2014-12-25
引用本文:
王蓓,黎源,陈贺,李桂欢,鲁义善,汤菊芬,蔡佳,吴灶和,简纪常. 我国华南地区罗非鱼源无乳链球菌分子流行病学研究[J]. 水产科学, 2014, (12): 791-799.
WANG Bei,LI Yuan,CHEN He,LI Gui-huan,LU Yi-shan,TANG Ju-feng,CAI Jia,WU Zao-he,JIAN Ji-chang. Molecular Epidemiology of Pathogen Streptococcus agalactiae from Tilapia in Southern China. Fisheries Science, 2014, (12): 791-799.
