Abstract：The drug-resistance was tested by Kirby-Bauer method ,and four drug-resistant genes including TEM ,ant(3 )-Ⅰ ,Sul3 and tet(A) genes against β-lactamase genotypes ,aminoglycoside ,sulfonamides and tetracyclines were detected in 35 strains (five strains each case) selected from 130 strains isolated from seven cases of Japanese flounder Paralichthys oliv aceus infected by Edw ardsiella tarda by PCR method to investigate the correlation between the drug resistance phenotypes and the drug resistance genotypes in the bacterial pathogen .The results showed that all detected strains had TEM and ant (3 )-Ⅰgenes ,62 .9%(22/35) of the strains carrying Sul3 gene ,and no tet(A) gene was detected in the experiment .All strains were found to be multi-drug resistant ,most of which were resistant to 7—9 antibiotics .There was a close relationship between the drug-resistance phenotype and the related resistance genes ,without entirely cor-responding .
收稿日期: 2015-05-25
引用本文:
葛慕湘,靳晓敏,张艳英,房海,陈翠珍. 迟钝爱德华氏菌耐药表型及 4 种耐药基因检测[J]. 水产科学, 2015, 34(5): 300-304.
GE Muxiang,JIN Xiaomin,ZHANG Yanying,FANG Hai,CHEN Cuizhen. Detection of Drug Resistance and Four Drug Resistance Genes in Bacterial Pathogen Edwardsiella tarda. Fisheries Science, 2015, 34(5): 300-304.
