Abstract:A strain of dominant bacterial HS1 was isolated from diseased yellow catfish Pelteobagrus f ul-vidraco ,then it was studied by biochemical characteristics ,16S rDNA gene sequences and drug sensitivity test .The results showed that HS1 was identified as obligate anaerobic ,power positive ,Gram -negative Cocci with optimum growth temperature of 28 ℃ .When the ethanol concentration was 2 .8% (v7) ,its growth was inhibited ,which resulted in an increase in turbidity and unpleasant sensory changes in beer . According to the analysis of 16S rDNA gene sequence ,the fragment size was 1466 bp with accession num-ber of KP191046 .Strain HS1 comprised sequences more related to Macrococcus caseolyticus (99% homol-ogy) ,which showed that strain HS1 is M .caseolyticus .Strain HS1 was sensitive to tetracyclines ,amin-oglycosides ,amphenicols ,quinolones ,andβ-lactams ,while it was resistant to sulfa-drugs .The findings provide a scientific reference with the prevention and treatment of M .caseolyticus infection in fish .
收稿日期: 2015-12-25
引用本文:
蓝胜华,王利,邓麟杰,杨启明,李雪峰,段荟芹. 黄颡鱼溶酪巨型球菌的鉴定与药敏试验[J]. 水产科学, 2015, 34(12): 778-782.
LAN Shenghua,WANG Li,DENG Linjie,YANG Qiming,LI Xuefeng,DUAN Huiqin. Identification and Drug Sensitivity of Megasphaera from Yellow Catfish Pelteobagrus fulvidraco. Fisheries Science, 2015, 34(12): 778-782.