Abstract：The offsprings of gynogenic Pengze crucian carp (Carassius auratus var .Pengze) (Pcc) is mono‐female groups theoretically .The previous studies showed that a high proportion of gynogenic male Pcc fish occurred in F 1 progenies in laboratory culture compared with pond culture condition ,and this phenomenon has been demonstrated in the high density groups of F2 progenies later .In the present study ,the different gene expression of Vtg B ,and ZP2 as female‐specific gene and Vtg contents were detected between male and female F 1 and F2 progenies .Results showed that testical Vtg B and ZP2 transcripts of F1 progenies were significantly higher than those in ovary under laboratory culture condition ;testical Vtg B and ZP2 transcripts of F1 progenies were significantly higher ,lower than those in ovary under pond culture condition ;and testical ZP2 transcript of F2 progenies was very significantly higher than those in ovary under laboratory culture condition .Male hepatic Vtg B and ZP 2 transcripts of F1 proge‐nies were very significantly higher ,lower than those in ovary under laboratory culture condition ;male hepatic Vtg B and ZP2 transcripts of F1 progenies were very significantly lower than those in ovary under pond culture condi‐tion;and male hepatic Vtg B and ZP2 transcript of F2 progenies was very significantly higher than those in ovary under laboratory culture .Male Vtg content of F1 progenies was very significantly lower than those in ovary under laboratory culture ;male gonadal and hepatic Vtg content of F1 progenies were very significantly higher ,lower than those in ovary under pond culture condition respectively ;and male gonadal ,hepatic Vtg content of F2 progenies was very significantly higher than those in ovary under laboratory culture condition .The findings referred that Vtg B and ZP2 were not female‐specific ,and Vtg B ,ZP2 transcripts and Vtg contents have been affected in various cul‐ture condition and stocking density .
