摘要在水温17～18℃和盐度30条件下，将初始体质量为（4．09±0．26） g的仿刺参饲养在15个循环水玻璃缸（容积100 L ）中，投喂在基础饲料中添加0％、3％、6％、9％和12％浒苔的饲料，于投喂后第7、14、28 d和42 d分别检测仿刺参的生长指标、消化酶（蛋白酶、淀粉酶、纤维素酶和褐藻酸酶）和体腔液免疫酶（溶菌酶、酸性磷酸酶、碱性磷酸酶和超氧化物歧化酶）的活性。试验结果表明，投喂试验饲料后第14、28 d和42 d ：（1）饲料中添加6％和9％浒苔组仿刺参的特定生长率显著增加（ P＜0．05），在不同取样时间其他添加组仿刺参的的特定生长率与对照组差异不显著（P＞0．05）；（2）饲料中浒苔添加量为6％和9％试验组仿刺参的4种消化酶比活力显著高于对照组（P＜0．05），其中6％组仿刺参的消化酶比活力最高；（3）饲料中浒苔添加量为6％时，仿刺参溶菌酶活力显著高于对照组及其他试验组（P＜0．05）；饲料中浒苔添加量为6％和9％时，仿刺参碱性磷酸酶活力和超氧化物歧化酶活力显著高于对照组及其他试验组（ P＜0．05）；添加浒苔可以显著提高酸性磷酸酶活力，至第42 d ，试验组酸性磷酸酶活力高于对照组（P＜0．05），且浒苔添加量为6％时活力最高。在本试验条件下，饲料中添加浒苔可以提高仿刺参的特定生长率、消化酶活力及免疫力，浒苔的最适添加量为6％～9％。
Abstract：A 6‐week feeding experiment was conducted to evaluate the effects of dietary green laver Entero‐mopha prolifera on growth ,activities of digestive enzymes and immunity in sea cucumber Apostichopus japonicus with initial body weight of (4 .09 ± 0 .26) g .The sea cucumber juveniles were reared in 15 tanks of each 100 L in volume with recycling water and fed the diets containing 0% ,3% ,6% ,9% and 12% of green laver powder at water temperature of 17—18 ℃ and a salinity of 30 .The specific growth rate ,and activities of digestive enzymes including protease ,amylase ,cellulase ,and alginase and immune enzymes including lysozyme ,acid phosphatase ,alkaline phosphatase and superoxide dismutase were analyzed 7 , 14 ,28 and 42 days after the sea cucumber were fed different diets .The results showed that there were sig‐nificantly increase in specific grow th rate in the sea cucumber fed the diets supplemented with 6% and 9%green laver on the days 14 ,28 and 42 (P<0 .05) ,without significant differences among other groups com‐pared with control group (P>0 .05) .Compared with the control group ,there were significantly higher ac‐tivities of the digestive enzymes in the sea cucumber fed the diets containing 6% and 9% of green laver (P<0 .05) ,the maximum in the sea cucumber fed the diets containing 6% of green laver (P<0 .05) .The sea cucumber fed the diet supplemented with 6% of green laver had significantly higher activities of lysozyme , acid phosphatase and superoxide dismutase than the juveniles in other groups (P<0 .05) .It is concluded that dietary green laver leads to significant increase in specific grow th ,activities of digestive enzymes and immunity of sea cucumber ,with the optimal supplementation of 6% —9% under the present experimental conditions .
收稿日期: 2016-05-25
引用本文:
杨宁,郭中帅,王正丽. 饲料中添加浒苔对仿刺参幼参生长、消化酶活性和免疫力的影响[J]. , 2016, 35(5): 498-503.
YANG Ning,GUO Zhongshuai,WANG Zhengli. Effects of Dietary Green Laver Enteromorpha proli fera on Growth,Activity of Digestive Enzymes and Immunity in Sea Cucumber Apostichopus j aponicus. , 2016, 35(5): 498-503.
