摘要根据克隆的黄鳝载脂蛋白A1基因的部分序列，进行5′RACE扩增和内含子的克隆并采用荧光定量PCR分析该基因的表达谱。结果表明，该基因cDNA全长1207 bp ，5′UTR区域长32 bp ，编码1个262 aa的多肽；在长1391 bp的gDNA上，只发现了1个长184 bp的内含子。荧光定量PCR对该基因在不同组织和病原细菌嗜水气单胞菌感染后的表达情况分析表明，该基因转录本在肝脏中表达量最高，在胃、肾脏、小肠、脑、皮肤和血液中表达量中等，而在心脏、脾脏和肌肉中表达量很低；病原细菌感染会显著影响该基因在小肠、肝脏和脾脏中的表达水平。以上结果显示黄鳝的载脂蛋白 Apo‐A1基因可能参与了鱼体的天然免疫反应。
Abstract：In this paper ,full‐length cDNA and genomic structure of Apo‐A1 gene were identified based on the cDNA fragment that we previously reported .Tissue distribution and immune response of the Apo‐A1 gene to bacterial challenge were investigated .Results showed that the full‐length cDNA was 1207 bp in length including a 32 bp‐long 5′untranslated region (UTR) encoding a 262 amino acids .In the 1391 bp‐long genomic DNA ,just one intron was identified .Real‐time quantitative PCR demonstrated that Apo‐A1 transcripts were ubiquitously expressed in ten tissues ,with distinctly different expression level .Signifi‐cant changes were observed in intestine ,liver and spleen after the fish was challenged with pathogenic bac‐terium Aeromonas hydrophilia .The findings suggeste that Apo‐A1 may involve in the immune system of this fish .
收稿日期: 2016-06-25
引用本文:
阚延泽,江翱,孙文秀,李伟. 黄鳝载脂蛋白 A1的基因结构及表达谱分析[J]. , 2016, 35(6): 697-701.
KAN Yanze,JIANG Ao,SUN Wenxiu,LI Wei. Gene Structure and Expression of Apo-A1 Gene f rom Swamp Eel. , 2016, 35(6): 697-701.
