Abstract：The enzyme production of a bacterium strain with relatively high alginate lyase activity known as Acinetobacter X8 isolated from decaying part of seeweed Sargassum fusiform was investigated under liquid fermentation. The optimized liquid fermentation medium(m/V) was comprised of 0.6 % alginate, 0.7 % peptone, 0.2 % K_2HPO_4, and 2 % NaCl at initial pH of 7.0. After fermentation for 16 h at 150 r/min and 25 ℃, the yield of alginate lyase was found to be up to 157.32 U/ml, 159.01% higher than that under the ordinary conditions. However, the production process was inhibited by Mg~2, Ca~(2+) and Fe~(2+).
收稿日期: 2009-11-25
引用本文:
侯保兵,刘书来,张建友,包华芳,丁玉庭. 褐藻胶裂解酶产生菌的发酵优化研究[J]. 水产科学, 2009, 28(11): 667-670.
HOU Bao-bing,LIU Shu-lai,ZHANG Jian-you,BAO Hua-fang,DING Yu-ting. Optimization of Alginate-degrading Bacterial Fermentation Process for Alginate Lyase Production. Fisheries Science, 2009, 28(11): 667-670.
