Abstract:A total of 39 broodstock of 4 to 5 year old Brycon cephalus(female with body weight of 2.5~2.9 kg and body length of 36~41 cm; male with body weight of 1.5~1.6 kg and body length of 32~35 cm)was induced to spawn by two hormonal injection at an interval of 6~7 hours at a rate of HCG 300 IU +LHR-A_2 2.5 μg + DOM 2 mg/kg,HCG 3500 IU+PG 2 mg+LHR-A_2 3 μg +DOM 3 mg/kg,PG 4 mg+LHR-A_2 6 μg+DOM 2 mg/kg, HCG 700 IU+DOM 2 mg/kg, and LHR-A_2 5 μg+DOM 4 mg/kg at water temperature of 26~28 ℃ from 2007 to 2008. There were 75.2%, 75.4%, 75.5%, 60% and 60.3% of the broodstock which spawned 9~10 hours after the injection with fertilization success of 76.6%. Total of 59560 fertilized eggs and 23469 larvae(incubation period of 17~18 h) were obtained with hatching rate of 68.5%.