Abstract：From November 2018 to May 2019, during the investigation of fish parasites in the markets and farms around rümqi City, it was found that there were a large number of Ancyrocephalidae in the gills of tilapia in different regions. After dye observation,the structure of the copulatory organs and haptors were mapped, measured and compared. At the same time, three molecular markers (28S, 18S-ITS1-5.8S and COⅠ) were selected for amplification, cloning and sequencing and phylogenetic analysis was carried out with ribosomal RNA and related species. The results showed that the morphological characteristics preliminarily identified the anchored worm as Ancyrocephalidae, Cichlidogyrus sclerosus (MJ 12) and C. mbirizei (MJ 23 or MJ 33) in Cichlidogyrus. The 28S, 18S-ITS1-5.8S and COⅠ sequence fragment size of MJ12 were 863 bp, 1019 bp and 592 bp, respectively, the similarities with C. sclerosus published in Genbank were above 99.5%; the 28S, 18S-ITS1-5.8S and COⅠsequences of MJ23 were 863 bp, 1005 bp and 572 bp, respectively, the similarity between MJ23 and C. mbirizei in GenBank was over 94%, and the similarity between MJ23 and C. cirratus was over 99.3%. According to the ML tree and genetic matrix constructed based on ribosomal RNA, MJ12 and Chitin cichlids from different regions are clustered together, and the genetic distance was 0; the topological structure formed by MJ 23 or MJ 33 and the C. mbirizei in Brazil and the C. cirratus had a self-expanding value of 99% and 100%, with genetic distance of 0.000—0.003, consistent with the morphological result.The findings supplemented the domestic molecular data of the worm, and also supported the viewpoint of the classification of synonymy species of C. mbirizei and C. cirratus.
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2021, Vol. 40 
