Genomic Characteristics of Virulence Factors of Aeromonas salmonicida Strains from Distinct Sources
HAO Jingwei1,2, WANG Yi3, HE Fenglan4, FU Songzhe1,2, SHEN Xudong1,2,5, LIU Ying1,2
1. College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China; 2. Key Laboratory of Environment Controlled Aquaculture, Ministry of Education, Dalian Ocean University, Dalian 116023, China; 3. School of Marine Resources and Environment, Hebei Normal University of Science & Technology, Qinhuangdao 066000, China; 4. Nanchang Center for Disease Control and Prevention, Nanchang 330038, China; 5. Yingkou Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Yingkou 115004, China
Abstract：A pathogenic Aeromonas salmonicida strain YK48 was isolated from the water of the Pacific white shrimp culture ponds. We first tested the physiological characteristics and antimicrobial susceptibility of strain YK48. Afterwards, to obtain its virulence profile, strain YK48 was sent for whole-genome sequence. Together with one strain isolated from goldfish Carassius auratus in Anshan and another two isolated from Yantai, a comparative genomic analysis was conducted to determine whether the strains had distinct virulence gene profile and antibiotic resistance genes. Phylogenetic analysis revealed that AS.17 and YK-48 were clustered together with Aeromonas salmonicida ssp. salmonicida strain A449; antimicrobial susceptibility test showed that strain YK-48 was resistant to rifampicin, bacitracin, vancomycin and penicillin; comparative genome analysis showed that the virulence genes of strains AS.17 and YK-48 were basically identical. Compared with S68 and S121, YK-48 and AS.17 lacked the genes related to anti-phagocytosis and immune regulation. Adherence assays also verified that strain YK-48 and AS.17 had relative weak adhesion ability. At present, there was comparative genomic analysis on the virulence factors of different A. salmonicida from distinct sources. The A. salmonicida strains from three sources were compared through comparative genomics, which provided valuable information regarding the pathogenicity of A. salmonicida, and provided useful insights into the prevention and control of the disease.
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2022, Vol. 41 
