Abstract：Feeding analysis of edible jellyfish Rhopilema esculentum is helpful to understand its food sources and functional role. Comprehensively and accurately obtaining the information on food species is critical for this endeavor.However, information about the feeding types and food structures of jellyfish remains obscure because of the limitations of traditional methods. Unrestricted to the morphology of prey, DNA barcoding based method could get more accurate results efficiently. DNA Barcoding is not only used in initially proposed simple species identification, but also in studies of animal diets, food chain, and food web by using short DNA fragments extracted from feces and intestinal contents of animals.In this study, DNA barcoding method was used to analyze the diet of jellyfish in Liaodong Bay coastal waters to make more comprehensive analysis towards its diet compositions. COⅠ gene and ITS-5.8S rDNA were used as the target gene, and universal primers were designed to identify food composition of the jellyfish by DNA barcoding. A total of 36 effective COⅠ gene sequences were obtained and 4 species were identified, while 10 species from 30 effective ITS-5.8S rDNA sequences were identified by traditional sequencing. The results showed that using DNA barcoding identified some potentially food compositions species of the jellyfish, such as fish eggs/larvae, and planktonic larvae of shellfish. Howerer, those main preys of plankton were not found in traditional Sanger Sequencing on the dietary analysis of the jellyfish yet, which means the deficiency of Sanger Sequencing in analyzing food composition research. The finding provides reference for further study of jellyfish feeding.
[1]PETHYBRIDGE H R, CHOY C A, POLOVINA J J, et al. Improving marine ecosystem models with biochemical tracers[J].Annual Review of Marine Science,2018,10:199-228. [2]SHEPPARD S K, HARWOOD J D. Advances in molecular ecology:tracking trophic links through predator-prey food-webs[J].Functional Ecology,2005,19(5):751-762. [3]张芳,孙松,李超伦.海洋水母类生态学研究进展[J].自然科学进展,2009,19(2):121-130. [4]金鑫,李超伦,刘梦坛.基于脂肪酸标记法和碳氮稳定同位素比值法的东海水母常见种的食性分析[J].海洋与湖沼,2012,43(3):486-493. [5]丁军军,徐奎栋.黄海水母旺发区浮游鞭毛虫和纤毛虫群落结构分布及其与水母发生关系初探[J].海洋与湖沼,2012,43(3):527-538. [6]孙明,王彬,李玉龙,等.基于碳氮稳定同位素技术研究辽东湾海蜇的食性和营养级[J].应用生态学报,2016,27(4):1103-1108. [7]张健,王佚兮,冯慧敏,等.基于脂肪酸标记法和稳定同位素技术的通州湾养殖水域海蜇食性分析[J].南方水产科学,2021,17(1):25-31. [8]徐盛楠,孙婷婷,彭赛君,等.基于高通量测序技术的两种水螅水母现场食物研究[J].应用海洋学学报,2020,39(1):49-56. [9]陈丽华,陈钢,李少菁,等.厦门港球型侧腕水母(Pleurobrachia globosa)的摄食研究[J].厦门大学学报(自然科学版),2003,42(2):228-232. [10]UNDERWOOD A H, SEYMOUR J E. Venom ontogeny, diet and morphology in Carukia barnesi, a species of Australian box jellyfish that causes Irukandji syndrome[J].Toxicon,2007,49(8):1073-1082. [11]PURCELL J E. Predation on zooplankton by large jellyfish, Aurelia, Cyanea and Aequorea, in Prince William Sound, Alaska[J].Marine Ecology Progress Series,2003,246:137-152. [12]RIASCOS J M, VILLEGAS V, PACHECO A S. Diet composition of the large scyphozoan jellyfish Chrysaora plocamia in a highly productive upwelling centre off northern Chile[J].Marine Biology Research,2014,10(8):791-798. [13]HEBERT P D N, GREGORY T R. The promise of DNA barcoding for taxonomy[J].Systematic Biology,2005,54(5):852-859. [14]HEBERT P D N, CYWINSKA A, BALL S L, et al. Biological identifications through DNA barcodes[J].Proceedings of the Royal Society of London Series B:Biological Sciences,2003,270(1512):313-321. [15]VALENTINI A, POMPANON F, TABERLET P. DNA barcoding for ecologists[J].Trends in Ecology & Evolution,2009,24(2):110-117. [16]Pompanon F, Deagle B E, Symondson W O C, et al. Who is eating what:diet assessment using next generation sequencing[J].Molecular Ecology,2012,21(8):1931-1950. [17]ZINGER L, BONIN A, ALSOS I G, et al. DNA metabarcoding—need for robust experimental designs to draw sound ecological conclusions[J].Molecular Ecology,2019,28(8):1857-1862. [18]ALBERDI A, AIZPURUA O, BOHMANN K, et al. Promises and pitfalls of using high-throughput sequencing for diet analysis[J].Molecular Ecology Resources,2019,19(2):327-348. [19]LERAY M, YANG J Y, MEYER C P, et al. A new versatile primer set targeting a short fragment of the mitochondrial COⅠ region for metabarcoding metazoan diversity:application for characterizing coral reef fish gut contents[J].Frontiers in Zoology,2013,10(1):1-14. [20]席晓晴,鲍宝龙,章守宇.DNA条形码在鱼类胃含物种类鉴定中的应用[J].上海海洋大学学报,2015,24(2):203-210. [21]席晓晴,鲍宝龙,章守宇.DNA条形码在马鞍列岛海域皮氏叫姑鱼胃含物鉴定中的应用[J].水产学报,2017,41(10):1533-1541. [22]刘梦娜,徐磊,王雪辉,等.基于DNA条形码研究中国枪乌贼和鸢乌贼的食物种类组成[J].热带海洋学报,2020,39(4):61-69. [23]董婧,姜连新,孙明.渤海与黄海北部大型水母生物学研究[M].北京:海洋出版社,2013. [24]李玉龙,王彬,王文波,等.基于COⅠ基因分析辽东湾海蜇群体遗传多样性[J].水产科学,2016,35(4):404-409. [25]李云峰,李玉龙,周遵春,等.我国北方地区海蜇池塘养殖技术研究进展[J].水产科学,2020,39(2):286-291. [26]狄明玉,周遵春,李云峰,等.海蜇4个自然群体遗传多样性的微卫星标记分析[J].水产科学,2018,37(6):762-768. [27]刘春洋,王彬,李轶平,等.海蜇不同生长阶段的摄食方式和摄食习性[J].水产科学,2011,30(8):491-494. [28]FOLMER O, BLACK M, HOEH W, et al. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit Ⅰ from diverse metazoan invertebrates[J].Molecular Marine Biology and Biotechnology,1994,3(5):294-299. [29]KI J S, HWANG D S, SHIN K, et al. Recent moon jelly (Aurelia sp.1) blooms in Korean coastal waters suggest global expansion:examples inferred from mitochondrial COⅠ and nuclear ITS-5.8S rDNA sequences[J].ICES Journal of Marine Science,2008,65(3):443-452. [30]高小迪,陈新军,李云凯.水生食物网研究方法的发展和应用[J].中国水产科学,2018,25(6):1347-1360. [31]RATNASINGHAM S, HEBERT P D N. BOLD: The barcode of life data system (http:∥www.barcodinglife.org)[J]. Molecular Ecology Notes,2007,7(3):355-364. [32]DUPONT S, WILSON K, OBST M, et al. Marine ecological genomics:when genomics meets marine ecology[J].Marine Ecology Progress Series,2007,332:257-273. [33]STMAN C. Abundance, feeding behaviour and nematocysts of scyphopolyps (Cnidaria) and nematocysts in their predator, the nudibranch Coryphella verrucosa (Mollusca)[J].Hydrobiologia,1997,355(1/2/3):21-28. [34]马喜平,凡守军.水母类在海洋食物网中的作用[J].海洋科学,1998,22(2):38-42. [35]ZHAN A B, BAILEY S A, HEATH D D, et al. Performance comparison of genetic markers for high-throughput sequencing-based biodiversity assessment in complex communities[J].Molecular Ecology Resources,2014,14(5):1049-1059. [36]王敏晓,程方平,李超伦,等.基于线粒体cox1片段序列的胶州湾浮游动物DNA条形码分析[J].海洋与湖沼,2011,42(5):702-710. [37]MACHIDA R J, HASHIGUCHI Y, NISHIDA M, et al. Zooplankton diversity analysis through single-gene sequencing of a community sample[J].BMC Genomics,2009,10(1):1-7. [38]BUCKLIN A, ORTMAN B D, JENNINGS R M, et al. A “Rosetta Stone” for metazoan zooplankton:DNA barcode analysis of species diversity of the Sargasso Sea (Northwest Atlantic Ocean)[J].Deep Sea Research Part Ⅱ:Topical Studies in Oceanography,2010,57(24/25/26):2234-2247. [39]林先智,胡思敏,刘胜,等.传统测序与高通量测序在稚鱼食性分析中的比较[J].应用生态学报,2018,29(9):3093-3101. [40]孙鹏,凌建忠,张辉,等.基于高通量测序的象山港海域黑鲷(Acanthopagrus schlegelii)食性分析[J].生态学报,2021,41(3):1221-1228.
2022, Vol. 41 
