Genetic Differentiation of Shortjaw Tapertail Anchovy Coilia brachygnathus Population Based on Cytb and Morphology
WANG Weixin1, LI Yu1, ZHU Honggeng1, ZHANG Wanru1, FENG Guangpeng2, CHEN Jianhua1
1. Jiangsu Key Laboratory of Marine Biotechnology, School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005,China; 2. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Abstract：To investigate the population genetic differentiation of shortjaw tapertail anchovy Coilia brachygnathus in Poyang Lake and Hongze Lake, the genetic diversity of shortjaw tapertail anchovy populations in Duchang (DC) and Ruihong (RH) waters of Poyang Lake, and Gaoliangjian (GLJ) and Linhuai (LH) waters of Hongze Lake was studied by using morphometry and mitochondrial cytochrome b (Cytb) sequences. Clustering analysis of 26 trait parameters showed that DC population and RH population first clustered into one branch, and then clustered with LH population and GLJ population. The total contribution rate of the first three principal components in principal component analysis was 65.12%. The results of discriminant analysis showed that the discriminant accuracy of DC, RH, GLJ and LH populations were 91.40%, 80.00%, 84.60% and 82.50%, respectively. The above morphological analysis showed that the morphological differences of populations in the same lake area were small, but there were great morphological differences among different lakes. Genetic analysis of Cytb sequence showed that there were 33 haplotypes in 60 sequences, with total gene polymorphism (Hd) of 0.957±0.013, and the total nucleotide polymorphism (Pi) of 0.00462±0.00047. The genetic differentiation index (Fst) of the four populations was 0.00362—0.60873, and the gene flow was 0.16069—68.81077. The results of neutral test showed that the D and FS values of the four populations were negative. The phylogenetic trees constructed by haplotype distribution and genetic distance were divided into two branches: Poyang Lake and Hongze Lake. The findings indicated that there was significant genetic differentiation in the population of shortjaw tapertail anchovy between the two lakes.
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2022, Vol. 41 
