Comparative Analysis on Intestinal Microflora of Wild and Cultured Tench Tinca tinca
QIAN Yi1, XU Qinyu1, QIAN Long2, AI Tao2, XIANG Wei2, HU Bolin2, WANG Jun1, WANG Chenghui1
1. Shanghai Engineering Research Center of Aquaculture, National Demonstration Centre for Experimental Fisheries Science Education, Key Laboratory of Freshwater Aquatic Genetic Resources Certificated by Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; 2. Fisheries Technology Extension Station, Xinjiang Production and Construction Corps, Ürümqi 830002, China
Abstract：The bacterial community and function of the intestinal microbiota play important roles in the growth and development of fish. Tench Tinca tinca is a cultured fish species with high economic value in Xinjiang area, China. In order to compare the structure and abundance of intestinal microbial communities between the wild and cultured tench, the 16S rRNA gene (V3—V4 region) was sequenced between wild and cultured tench using Illumina Miseq high-throughput sequencing method. The results showed that the dominant intestinal microbial flora in tench was mainly Proteobacteria, Firmicutes, Actinobacteriota, and Bacteroidota. However, significant intestinal microbiota was identified between wild and cultured tench. In detail, at phylum level, the abundance of Fusobacteriota and Acidobacteriota in the cultured tench was significantly lower than that in the wild tench (P<0.05). At genus level, the abundance of Lactobacillus, and Acinetobacter was higher in the cultured tench than that in the wild tench, and the abundance of Mycoplasmataceae and Flavobacterium were lower in the cultured tench (P＜0.01). At species level, the abundance of A. lwoffii in the cultured tench was higher than that in the wild tench (P＜0.01), while the unclassified Flavobacterium was significantly lower in the wild tench (P＜0.05). It was found that the community and abundance of the intestinal microbiome was significantly different between wild and cultured tench. Our findings provide basic data for the research of intestinal microbiome and cultivation management of tench in future.
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2023, Vol. 42 
