Development of Larva, Irreversible Starvation Point and Optimal Stocking Time of Phoxinus lagowskii Larva
REN Xingyue, LUO Xiaonian, DENG Shuping, DUAN Youjian, JIANG Yusheng, LI Jiao
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
Abstract：In order to study the developmental biological characteristics of Phoxinus lagowskii larva and optimal stocking time, microscopic observation of the P. lagowskii larval development, the larval starvation test and larva pond experiment were carried out. The observation of postembryonic development of larva showed that: under the condition of water temperature 16—18 ℃, at the beginning of their P. lagowskii length was (4.69±0.37) mm, yolk sac total length was (3.12±0.10) mm, 2 days post hatching (dph) the color of P. lagowskii eyespots became black, the digestive tract began versed at 5 dph, and the silver fish swim bladder began inflating one room at 6 dph, when the larval notochord began to bend and initial feeding was observed in the larvae at 7 dph, the yolk sac was completely exhausted at 9 dph, and the larvae showed second chamber of swim bladder at 12 dph, second bladder chamber aeration period at 21 dph, the larvae dorsal and anal fin emerged at 23 dph, at 27 dph larval ventral fins began to form, until at 35 dph, all fins were well developed in the larvae. The experiment of P. lagowskii irreversible starvation point results showed that: under 16—18 ℃, the P. lagowskii larvae first day of open mouth (at 7 dph) feeding rate was 36.36%. At 9 dph, the highest initial feeding rate was 85.71%, the irreversible starvation point was at 14 dph, the larvae of feeding rate was 40.91%. length (L) and age (t) von-bertalanffy growth formulaof juvenile fish before mouth open was L=8.0031[1-e-0.3139(t+2.8093)]. The optimal stocking time of P. lagowskii results showed that water temperature was 14.5—20.1 ℃, rotifer biomass keeps 8.30—18.58 mg/L, the highest survival rate and time of body mass growth appeared at 6 dph, the survival rate was (75.0±2.2)% and the larvae body mass reached (9.0±2.0) mg. Under the optimum total length of largest growth of timely for 8 dph, the minimum total length of the coefficient of variation was at 7.5 dph, minimum weight coefficient of variation of timely under the pond at was 8.5 dph. This experiment provides basic materials for post-embryonic development and optimal stocking time in P. lagowskii larva.
任星月, 骆小年, 邓舒萍, 段友健, 姜玉声, 李姣. 拉氏鱼岁仔稚鱼发育、饥饿不可逆点及适时下塘研究[J]. 水产科学, 2023, 42(4): 594-603.
REN Xingyue, LUO Xiaonian, DENG Shuping, DUAN Youjian, JIANG Yusheng, LI Jiao. Development of Larva, Irreversible Starvation Point and Optimal Stocking Time of Phoxinus lagowskii Larva. 水产科学, 2023, 42(4): 594-603.
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2023, Vol. 42 
