Flocculation and Fragmentation of Particulate Matter in Biofloc Culture Water by Ozone
CHENG Xiangyu1,2, LIU Xingguo2, CHEN Zhe1,2, XIAO Shuwen1,2, PAN Hengfeng1,2, ZHANG Wang1,2
1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 2. Institute of Fishery Machinery and Instruments, Chinese Academy of Fishery Sciences, Shanghai 200092, China
Abstract：In order to understand the effect of ozone on flocculation and fragmentation of suspended particulate matter in aquaculture wastewater, the ozone concentrations corresponding to 0.5, 1.0, 1.5, 2.0 and 2.5 mg/L were aerated by an ozone generator for 5, 10, 15, 20 and 25 s in a 1 L ozone reaction vessel at a water temperature of (29±1) °C, respectively. Each ozone concentration was oxidized with the raw water in the circulating water biofloc shrimp culture system in Sanmao Aquaculture Farm in Songjiang District, Shanghai for 1, 2, 3, 4 and 5 min, and each combination test was repeated 3 times. Immediately after the treatment, the water quality index, the concentration of total residual oxidant (the concentration of total residual oxidant determined immediately at the end of aeration) and the particle size distribution of suspended particulate matter were measured in the water samples.The results showed that different ozone concentration treatments caused significantly increase in the levels of ammonia nitrogen (NH+4-N) and nitrate nitrogen (NO-3-N) contents in the water (P<0.05),with significant decrease in nitrite nitrogen (NO-2-N) level in the water column (P<0.05). There was significant decrease in total suspended solids (TSS) in the water (P<0.05) with different concentrations of ozone treatment, and the gradual increase in the removal rate of TSS with the elevated ozone concentration, with the maximum removal rate of 23.15% at ozone concentration of 2.5 mg/L. The size distribution of suspended particles in the water was found to be changed significantly after the ozone was put into the culture water, with gradual increase in the flocculation efficiency with the increase in ozone concentration, and the maximum flocculation efficiency of 33.61% at the ozone concentration of 2.5 mg/L.The findings indicated that ozone effectively removed nitrite nitrogen and flocculated suspended particles in the water.It is recommended that treatment of aquaculture waterbe conducted at the suitable ozone concentration of 1.5 mg/L for 4 min.
[1] 农业农村部渔业渔政管理局.2021中国渔业年鉴[M]. 北京:中国农业出版社,2022:2-3. [2] BAO W J, ZHU S M, JIN G, et al. Generation, characterization, perniciousness, removal and reutilization of solids in aquaculture water:a review from the whole process perspective[J]. Reviews in Aquaculture,2019,11(4):1342-1366. [3] 陈进斌,苗英霞,邱金泉,等. 海水养殖废水处理技术研究进展[J]. 盐业与化工,2016,45(5):1-5. [4] 王钰钦,郑尧,钱信宇,等. 中国水产养殖尾水污染现状及净化技术研究进展[J]. 农学学报,2022,12(3):65-70. [5] 季明东. 海水循环水养殖系统悬浮颗粒物去除机制及其粒径分布特征研究[D].杭州:浙江大学,2020. [6] 杜明辉,王勇,高群丽,等. 臭氧微气泡处理有机废水的效果与机制[J]. 化工进展,2021,40(12):6907-6915. [7] 张成林,杨菁,张宇雷,等. 去除养殖水体悬浮颗粒的多向流重力沉淀装置设计及性能[J]. 农业工程学报,2015,31(增刊):53-60. [8] 章亚芳,魏林生,张金宝,等. 臭氧改善水产养殖水体质量的作用机理初探[J]. 江西水产科技,2010,4 (1):17-20. [9] DAVIDSON J, SUMMERFELT S, ESPMARKÅ M O, et al. Effects of ozone on post-smolt Atlantic salmon (Salmo salar) performance, health, and maturation in freshwater recirculation aquaculture systems[J]. Aquaculture,2021,533:736208. [10] ZHU H T, WEN X H, HUANG X. Pre-ozonation for dead-end microfiltration of the secondary effluent:suspended particles and membrane fouling[J]. Desalination,2008,231(1/2/3):166-174. [11] PARK J, KIM Y, KIM PK, et al. Effects of two different ozone doses on seawater recirculating systems for black sea bream Acanthopagrus schlegeli (Bleeker):removal of solids and bacteria by foam fractionation[J]. Aquacultural Engineering,2011,44(1):19-24. [12] GUÉRIN L, FRANCES C, LINÉ A, et al. Fractal dimensions and morphological characteristics of aggregates formed in different physico-chemical and mechanical flocculation environments[J]. Colloids and Surfaces A:Physicochemical and Engineering Aspects,2019,560:213-222. [13] SCHROEDER J P, REISER S, CROOT P L, et al. Acomparative study on the removability of different ozone-produced oxidants by activated carbon filtration[J]. Ozone:Science & Engineering,2011,33(3):224-231. [14] POWELL A, SCOLDING J W S. Direct application of ozone in aquaculture systems[J]. Reviews in Aquaculture,2018,10(2):424-438. [15] GESTO M, DE JESUS GREGERSEN K J, PEDERSEN L F. Effects of ozonation and foam fractionation on rainbow trout condition and physiology in a small-scale freshwater recirculation aquaculture system[J]. Aquaculture,2022,557:738312. [16] POWELL A, CHINGOMBE P, LUPATSCH I, et al. The effect of ozone on water quality and survival of turbot (Psetta maxima) maintained in a recirculating aquaculture system[J]. Aquacultural Engineering,2015,64:20-24. [17] SCHROEDER J P, KLATT S F, SCHLACHTER M, et al. Impact of ozonation and residual ozone-producedoxidants on the nitrification performance of moving-bed biofilters from marine recirculating aquaculture systems[J]. Aquacultural Engineering,2015,65:27-36. [18] PUMKAEW M, TAWEEPHITAKTHAI T, SATANWAT P, et al. Use of ozone for Vibrio parahaemolyticus inactivation alongside nitrification biofilter treatment in shrimp-rearing recirculating aquaculture system[J]. Journal of Water Process Engineering,2021,44:102396. [19] TEITGE F, PEPPLER C, STEINHAGEN D, et al. Water disinfection by ozonation has advantages over UV irradiation in a brackish water recirculation aquaculture system for Pacific white shrimp (Litopenaeus vannamei)[J]. Journalof Fish Diseases,2020,43(10):1259-1285. [20] 管崇武,张宇雷,宋红桥,等. 臭氧对循环水养殖水体水质的净化效果及机理研究[J]. 渔业现代化,2018,45(6):14-18. [21] 黄晓婷. O3-BAC工艺在封闭循环水产养殖水处理中的应用研究[D].广州:华南理工大学,2011. [22] 叶丙聪,吕布,周建聪,等. 2种氧化法对模拟水产养殖废水净化效果[J]. 广东海洋大学学报,2022,42(3):33-38. [23] DE VERA G A, GERNJAK W, WEINBERG H, et al. Kinetics and mechanisms of nitrate and ammonium formation during ozonation of dissolved organic nitrogen[J]. Water Research,2017,108:451-461. [24] 扶泽发. 臭氧在生物絮团南美白对虾养殖水处理中的应用研究[D].上海:上海海洋大学,2021. [25] 宋奔奔,倪琦,张宇雷,等. 臭氧对大菱鲆半封闭循环水养殖系统水质净化研究[J]. 渔业现代化,2011,38(6):11-15. [26] DAVIDSON J, GOOD C, WELSH C, et al. The effects of ozone and water exchange rates on water quality and rainbow trout Oncorhynchus mykiss performance in replicated water recirculating systems[J]. Aquacultural Engineering,2011,44(3):80-96. [27] TANGO M S, GAGNON G A. Impact of ozonation on water quality in marine recirculation systems[J]. Aquacultural Engineering,2003,29(3/4):125-137. [28] 宋德敬,尚静,姜辉,等. 蛋白质分离器中的不同臭氧浓度对工厂化养殖净水效果的试验[J]. 水产学报,2005,29(5):719-723. [29] 段姗杉,杨晨,宋协法,等. 循环水养殖系统微细悬浮颗粒的分布规律研究[J]. 渔业现代化,2021,48(2):22-28. [30] 刘金金,张玲玲,张玉平. 凡纳滨对虾养殖尾水悬浮物粒径分布研究[J]. 水产科学,2024,43(1):41-50. [31] RUETER J, JOHNSON R. The use of ozone to improve solids removal during disinfection[J]. Aquacultural Engineering,1995,14(2):123-141. [32] 郭建宁,张锡辉,胡江泳,等. 臭氧氧化对陶瓷膜超滤工艺降低饮用水中浊度的影响[J]. 环境科学学报,2013,33(4):968-975.
2024, Vol. 43 
