Abstract：The purpose of this experiment was to study the effects of enzyme-digested hydrolyzed porcine mucosa (EPM) replacing fish meal on the growth performance, apparent digestibility coefficient of nutrients and aquaculture water quality in ponds withhybird grouper Epinephelus fuscoguttatus ♀×E. lanceolatu ♂. A total of 450 hybird grouper [initial body weight (7.50±0.02) g] was divided into 5 groups, each with 3 replicates, and each replicate with 30 fish was fed iso-nitrogenous and iso-lipidic diets with EPM replacement rates of 0%(EPM 0), 9%(EPM 9), 18%(EPM 18), 27%(EPM 27), and 36%(EPM 36) fish meal, respectively for 8-week. The results showed that the weight gain rate and specific growth rate in the EPM 9 group were not significantly different from that in the EPM 0 group (P>0.05), but were significantly higher than those in the other groups (P<0.05). The apparent digestibility coefficients of dry matter, crude protein, calcium and phosphoruswere significantly higher in EPM 0 group than those in the other groups (P<0.05). There was no significant difference in ammonia nitrogen content in aquaculture water between EPM 0 and EPM 9 groups with a period of 1 to 21 h after feeding (P>0.05). Twenty-one hours after feeding, there was no significant difference in the content of nitrite nitrogen in aquaculture water between the groups (P>0.05). In summary, when the substitution of 9% fish meal with enzyme-digested hydrolyzed porcine mucosa will not result in negative impact on the growth performance of the hybird grouper and the ammonia nitrogen content in aquaculture water.
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2023, Vol. 42 
