Development of an Inactivated Aeromonas salmonicida Vaccine for Turbot Scophthalmus maximus
SHENG Dong, REN Xu, LI Fei, HUANG Luxi, LIU Yuxiao, YU Qinghua, ZHOU Weijia, YE Shigen, GUO Zhixin
1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2. Center for Fish Disease Prevention and Control, Dalian Ocean University, Dalian 116023, China
Abstract：To prevent furunculosis in turbot Scophthalmus maximus, pathogenic bacterium Aeromonas salmonicida (DLPJC01) was inactivated with formaldehyde in the bacterial solution with a volume fraction of 0.05%, 0.10%, 0.20%, 0.30% and 0.40%, and its safety was tested to prepare an inactivated vaccine. Subsequently, the healthy turbot with body weight of (95.5±9.7) g were divided into 4 groups, with 30 individuals in each group, and intraperitoneally injected with the inactivated vaccine (vaccine group), equal volume of normal saline (control group), chito-oligosaccharide and inactivated vaccine + chito-oligosaccharide, respectively. After 14 days of immunization, 109 cfu/mL of A. salmonicide was injected into the turbot to calculate the relative immune protection rate. The content of immunoglobulin M (IgM) and the activities of lysozyme (LZM) and superoxide dismutase (SOD) in turbot serum were detected, and the expression of immune-related genes (TLR2, TLR7, and MyD88) in muscle and liver tissues was detected by real-time quantitative PCR. There were significant increase in IgM and upregulation of MyD88, TLR2, and TLR7 in the vaccine group after 14 days (P<0.05), with peak IgM, and activities of LZM, and SOD in chitooligosaccharide groupd at 14 days. Immune protection rates were found to be 0% in the control group, 100% in the vaccine group, and 88.89% in both the chitooligosaccharide group and the vaccine + chitooligosaccharide group. An inactivated A. salmonicidal vaccine was successfully developed for turbot, which is significant for preventing furunculosis.
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2025, Vol. 44 
