Abstract:Ciliates Pseudocohnilemus persalinus and Uronema marinum were held in a 96-well cell culture plate containing 90 μL of solution of 3 compound Chinese herbal medicines including Compound Ⅰ: Mahonia, Phellodendron, Scutellaria barbata, Sophora flavescens, Stemona, and Areca; Compound Ⅱ: Rheum, Scutellaria, Chrysanthemum indicum, Fructus cnidii, pomegranate peel, and mume; and Compound Ⅲ: dice, dandelion, chrysanthemum, broccoli, Patrinia, Gentian, Houttuynia, and Artemisia annua, at a dose of 0, 5, 10, 15, 20, 25, and 30 mg/kg, and 10 μL of centrifuged ciliates solution containing the ciliates at a density of 103 individuals/mL to screen the Chinese herbal medicine formulas in vitro insecticidal test by observation of the ciliates under a light microscope. Blood biochemical and histopathological indices were determined and observed in juvenile turbot Scophthalmus maximus exposed to the 3 compound Chinese herbal medicines above at a dose of 0, 20, 60, and 100 mg/kg for 7 days to evaluate toxicity of the 3 compound Chinese herbal medicines to turbot. Killing effect was observed in U. marinum exposed to Compound Ⅲ at a dose of 30 mg/kg, without mortality in Compound I solution at a dose of 0—30 mg/kg. All ciliate P. persalinus was found to be dead in the compound Ⅱgroup for 1 hour at a dose of 20, 25, and 30 mg/kg, and U. marinum was all dead in the compound Ⅱgroup for 3 hour at a dose of 30 mg/kg. There were no significant differences in blood routine indicators including white blood cells and eosinophils, as well as blood biochemical indicators such as glucose and globulin, without obvious pathological and pathological changes in the intestine, stomach, liver and spleen in each test group.
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