Abstract：To determine the optimal stocking density for venus clam Cyclina sinensis and hard-shell clam Mercenaria mercenaria, venus clam with body weight of (8.19±0.25) g and hard-shell clam with body weight of (6.75±0.14) g were reared in a 1 m3 canvas tank of a recirculating water system with 1∶1 mixed sediment at the bottom at stocking densities of 200, 300, 400 and 500 ind./m2 and 500, 600, 700 and 800 ind./m2,respectively, at water temperature of (22±3) °C for 60 days. The results showed that there were a downward trend in both the growth rate and survival rate of venus clam with increase in stocking density, with significantly higher survival rate in 200 ind./m2 group than that in 400 ind./m2 and 500 ind./m2 groups (P<0.05), and without significant difference compared with those in 300 ind./m2 group (P>0.05). The body weight and specific growth rate (SGR) were found to be significantly higher in venus clam in 200 ind./m2 group than those in 400 ind./m2 and 500 ind./m2 groups (P<0.05), and significantly higher in 300 ind./m2 group than those in 500 ind./m2 group (P<0.05). Hard-shell clam had decrease in all growth indices with increasing density, without significant differences in SGR among density groups (P>0.05). However, the survival rate was shown to be decreased in hard-shell clam with increasing density, without significant differences in survival among groups (P>0.05). Throughout the culture period, key water quality parameters including ammonia nitrogen and nitrite nitrogen contents were remained within suitable ranges. The activities of pancreatic proteases and amylases were found to be gradually decreased in venus clam as stocking density increased, with significantly higher protease and amylase activities in 200 ind./m2 group than those in 400 ind./m2 and 500 ind./m2 groups (P<0.05). No significant differences in digestive enzyme activities were observed among hard-shell clam treatment groups (P>0.05), with the minimal protease and amylase activities in 700 ind./m2 group and the maximal in 500 ind./m2 group. Lipase activity was shown to be decreased in hard-shell clam with increasing density. Collectively, these results indicate that under short-term recirculating water culture conditions, stocking density of 300 ind./m2 for C. sinensis and 800 ind./m2 for M. mercenaria did not led to significant adverse effects on their growth performance, main digestive enzyme activities, and water quality.
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2026, Vol. 45 
