Abstract：In order to investigate acute toxicity of NO-2-N concentration on mortality, antioxidant capacity and nonspecific immunity of juvenile yellow drum Nibea albiflora under different salinities, yellow drum juveniles weighing (10±2) g were stocked in an indoor glass tank of 50 cm×50 cm×50 cm at water temperature of 25—27 ℃and salinity of 26—28. Then salinity was adjusted at a rate of 2 per day with aerated tap water and sea crystals to gradually adjust the water salinity to 15, 20, 25, 30, 35 and 40. After 7 d of adaptation, an appropriate amount of NaNO2 was added to adjust the NO-2-N concentration to the expected value from 9.85 mg/L to 280.21 mg/L. The results showed that the median-lethal concentration of NO-2-N to juvenile yellow drum showed a trend of increasing first and then decreasing under the same stress time ( 24, 48, 72 h and 96 h) with the increase in salinity from 15 to 40, with the strongest tolerance of juvenile yellow drum to NO-2-N at the salinity of 35. The juvenile yellow drum had first increase in activities of serum superoxide dismutase and then decreased at salinity of 15 with the elevated NO-2-N concentration and the extension of exposure time, with decrease and increase in activities of superoxide dismutase at salinity of 25, 35 and 40. There were decrease in activities of serum catalase, alkaline phosphatase and lysozyme in juvenile yellow drum and increase in content of malonaldehyde with the increasing NO-2-N concentration and the prolongation of exposure time. The safe concentration of NO-2-N for juvenile yellow drum was found to be 6.00 mg/L at salinity of 15, 6.89 mg/L at salinity of 20, 13.45 mg/L at salinity of 25, 16.86 mg/L at salinity of 30, 22.29 mg/L at salinity of 35 and 19.12 mg/L at salinity of 40. The findings indicate that salinity can affect the toxicity of NO-2-N, the juvenile yellow drum having the strongest tolerance to NO-2-N at salinity of 35. Under the same salinity, the decrease in antioxidant capacity and non-specific immunity and increase in the oxidative damage were observed in juvenile yellow drum, with the increase in NO-2-N concentration and the extension of exposure period in aquaculture water.
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2025, Vol. 44 
