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| Anesthetic Efficacy of Two Anaesthetics on Juvenile Chinese Hook Snout Carp Opsariichthys bidens |
| ZHOU Ye, LUO Xiaonian, DUAN Youjian, LI Jiao, LUO Laixing, ZENG Xue, LIU Jiaxin |
| College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China |
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Abstract In order to reduce the high mortality of Chinese hook snout carp Opsariichthys bidens due to stress during artificial reproduction and transportation, the Chinese hook snout carp with body weight of (99.17±7.43) g were held in a 3.0 m× 2.5 m× 1.5 m tank with water depth of 0.7 m, and exposed to MS-222 at concentrations of 10, 15, 20, 25, 30, 35 and 40 mg/L and 2-phenoxyethanol at concentrations of 0.18, 0.22, 0.26, 0.30, 0.34, 0.38 and 0.42 mL/L at water temperature of (12.0±0.5) ℃ to determine the optimal anesthetic dose with a survival rate of 100% in 15 min of anesthesia with 3 min into anesthesia and recovery in 5 min. The effects of water temperature on the anesthetic effect of these two anesthetics were investigated at the concentrations of 35 mg/L MS-222 and 0.34 mL/L 2-phenoxyethanol at water temerature of (12.0±0.5), (15.0±0.5), (18.0±0.5), (21.0±0.5) and (24.0±0.5) ℃. After the fish entered the deep anesthesia (A5) phase under the concentrations of the two anesthetics, they were exposed to air for 0, 2, 4, 6, 8, 16 and 24 min, respectively, and then put back into clean water for recovery to evaluate the effect of anesthesia on air exposure. Anesthesia under 7 mg/L MS-222 and 0.10 mL/L 2-phenoxyethanol, the fish was simulated to the long-distance transportation in still water in a sealed and oxygenated plastic bag at water to fish weight ratio of 1∶25, 1∶20 and 1∶15 to test the final anesthesia state and behavioral characteristics during recovery. The results showed that there was the effective mass concentration of 30—35 mg/L MS-222 at (12.0±0.5) ℃, with the enhanced anaesthetic effect with the increasing concentration, and significant difference between all groups (20—40 mg/L) (P＜0.05). The Chinese hook snout carp was deeply anaesthetised by MS-222 at the optimal mass concentration of 35 mg/L with recovery rate of 100% in the fish with air exposure for 8 min. The Chinese hook snout carp was anaesthetized by 2-phenoxyethanol at 3 min and recovery at 5 min with an effective volumetric concentration of 0.30 to 0.38 mL/L, with the enhanced anaesthesia effect with increasing volume concentration, and significant difference among all groups (0.26—0.42 mL/L) (P< 0.05). Chinese hook snout carp deeply anaesthetised by the optimal volume concentration of 0.34 mL/L 2-phenoxyethanol had a recovery rate of 92% with air exposure for 8 min. The anaesthetic effects of the two anaesthetics were shown to be closely related to the water temperature, and the anaesthetic effects were increased with the increase in water temperature. The best 24 h transportation effects were found at 7 mg/L MS-222 and 0.10 mL/L 2-phenoxyethanol at a fish-water ratio of 1∶20. The findings provide technical support for improvement of the efficiency of artificial reproduction and culture and transport survival of Chinese hook snout carp via screening the appropriate anaesthetic drugs and dosages.
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Received: 30 August 2023
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