Fisheries Science

Office Online

Journal Online

Download

Information

月刊，1984年创刊
主管：内蒙古自治区教育厅
主办：包头医学院
主编：赵云山
特邀主编：高长青
编辑出版：包头医学院学报编辑部
国际刊号：ISSN 1006－740X
国内刊号：CN 15－1182/R
邮发代号：16-292

Links

2015 Vol. , No. 10 Published: 2015-10-25

Article

	597	Effect of Salinity Stress on Physiological Status and Survival in Hybrid Sturgeon
		WANG Xiaowen,ZHU Hua,KUANG Xinyu,HU Hongxia
		To investigate the effects of abrupt salinity changes on survival rates and physiological status , hybrid sturgeon (Siberian huso sturgeon H uso dauricus♀ × Amur sturgeon A cipenser schrenckii ♂) ju‐veniles with body weight of(7 .28 ± 0 .56) g were transferred to freshwater (control) and salinity levels of 5 ,10 ,15 ,and 20 .The serum non‐specific immune indicators ,activities of antioxidant enzymes in liver and activities of Na+ /K+‐ATPase in gill were analyzed 1 ,6 ,12 ,24 ,48 ,and 96h after salinity change . The results showed the fish exposed to a salinity of 20 were all found dead within one hour ,exposed to sa‐linity of 10 ,and 15 all dead in 96h and 12 h ,respectively ,w hile no mortality occurred in salinity 5 group and control groups .Activities of gill Na+ /K+‐ATPase in salinity 5 group increased sharply after immer‐gence ,the serum total protein level were at peak at 12h ,and serum acid phosphatase (AKP) activity in‐creased slightly(P> 0 .05) .While there were no significant differences between salinity 5 and control groups except for peak at 48 h(P<0 .05) .In salinity 10 group ,the activities of serum lysozyme signifi‐cantly rose at 6 h ,and AKP activity reached a maximum at 24h after that decreased dramatically ( P<0.05) .Obviously ,the activities of serum alanine aminotransferase (ALT)in salinity 10 group were signif‐icantly higher than those in salinity 5 or control groups except the activity at 12 h .In salinity 15 group ,ac‐tivities of serum lysozyme significantly rose at 6h(P<0 .05) ,at the same time ,the serum total protein de‐clined rapidly .The activities of superoxide dismutase (SOD) and contents of malonaldehyde (MDA) in liv‐er in all groups showed the same trend as firstly increasing ,then decreasing ,after that slightly rising ,and the minimum at 12 .Also ,the range of changes in salinity 10 group was greater than in salinity 5 group . Activities of liver catalase(CAT)in each group were decreased to the minimum at 1 h(P<0 .05) ,then ele‐vated to the maximum at 12h ,and decreased continuously .
		2015 Vol. (10): 597-602 [Abstract] ( 101 ) HTML (0 KB) PDF (1338 KB) ( 300 )

	603	Analysis and Discrimination of Sexual Dimorphism of Hemibarbus maculatus Bleeker by Morphometry
		TUO Yun,XIAO Tiaoyi
		Hemibarbus maculates Bleeker ,a small freshwater fish in family Cyprinidae ,as an endemic species in China ,is of great commercial value .In this paper ,we studied morphological information of 132 H .maculates samples collected at Yuanzhou section (113°54′-114°37′E ,27°33′-28°05′N )of Yuanhe River in Yichun , Jiangxi Province from March in to 2009 June in 2012 .Based on the analysis of the morphological index system by Principal Components and R-cluster Analysis ,H .maculates was classified by body size and condition factor .On the basis of this analysis ,19 characteristics were normalized and further used for stepwise discriminant ,and then 5 characteristics were screened including condition factor , interorbital ,body depth ,distance between pelvic fin and anal fin ,and caudal peduncle length .Thus the discriminant equations were built for classification of 132 samples with accuracy rate of 97% .One‐way ANOVA revealed that there were significant differences in seven standardized characteristics and body length between male and female populations (P<0 .05) .Therefore ,it was concluded that the female H . maculates collected from Yuanhe River were superior to males in the condifion factor and body size ,but had smaller interorbital diameter ,caudal peduncle length and pectoral fin length .The findings can be used in sexual identification of H .maculatus and provides the basis information for its reproduction .
		2015 Vol. (10): 603-608 [Abstract] ( 63 ) HTML (0 KB) PDF (1081 KB) ( 315 )

	609	Effects of Temporal,Spatial and Environmental Factors on Distribution of Antarctic Krill (Euphausia superba) in 2013 Based on Generalized Additive Model

		According to the data of Antarctic krill (Euphausia superba) trawl survey from January to September in 2013 ,the temporal and spatial distribution of Antarctic krill resources were analyzed with the index of CPUE .The results showed that monthly average catch per unit effort (CPUE) was relatively stable from January to June ,but declined after July .The maximum(25 .12 ± 31 .04) t/h average CPUE was observed in subarea 48 .1 ,and the minimum (11 .49 ± 12 .06) t/h in subarea 48 .3;whereas ,the change in net CPUE was greater in subarea 48 .1 than in 48 .2 and 48 .3 .The results of stepwise GAM showed that the important explanatory variables affecting the spatial distribution of Antarctic krill were month ,longitude ,trawling depth ,sea bottom depth ,latitude and surface sea temperature (SST) ,and the deviance explanation by these explanatory variables was accounted for 36 .14% in total .
		2015 Vol. (10): 609-615 [Abstract] ( 58 ) HTML (0 KB) PDF (2000 KB) ( 245 )

	616	Comparisons of Phenotypic Traits in Two Shell Type Strains of Manila Clam in Zhangzi Island

		Two shell type strains “wide‐shell” and “plate‐shell” of Manila clam ( Ruditapes philippenarum) were established using Zhangzi island populations .In this experiment ,the shell length , shell width ,shell height ,live body weight ,tissue weight and fecundity were compared between these two strains .The growth and survival of the offsprings were studied .The results showed that the wide‐shell strain had significant higher shell length ,live body weight and tissue weight than plate‐shell strain did (P<0 .05) .The shell width which had the maximal direct effect was the key effective factor on live body weight for both strains .The shell length which had the maximal direct effect was the key effective facter on body‐tissue weight for both strains .No significant difference was observed in D‐larvae at days 0 ,3 and 6 between the two strains (P> 0 .05) ,but plate‐shell strain had larger shell length than the wide‐shell strain did (P<0 .05) at 15 days .At juvenile stage ,the shell length of wide‐shell strain was larger than the plate‐shell strain (P<0 .05) except at 30 days .The wide‐shell strain had higher survival than the plate‐shell strain at larval and juvenile stages (P<0 .05) .
		2015 Vol. (10): 616-620 [Abstract] ( 56 ) HTML (0 KB) PDF (822 KB) ( 292 )

	621	Characteristics of Flora Community in Sediment in Red Swamp Crayfish Procambarus clarkii Ponds with Different Stocking Density and Aquatic Vegetation
		QIN Wei,ZHOU Xin,XU Zenghong,LIU Guofeng,ZHANG Ping,BAI Aixu
		The composition and diversity of flora community were studied in sediments in red swamp crayfish Procambarus clarkii ponds with biological bait medium bottom with two kinds of aquatic vegetation (20% and 35% of water area ,group A ,and group B) and stocking densities (375 kg/hm2 and 600 kg/hm2 ,group C ,and group D) with duplication by PCR‐DGGE and DNA sequencing technology .It was found that there were different bacterial species and count in the sediments in the four treatment groups with Shannon index of 2 .72 in group A ,2 .94 in group B ,2 .82 in group C and 2 .96 in group D , the most abundant in group D .UPGMA analysis revealed that low similarity of microbial community structure was observed in the different sediments in different time ,the higher similarity (70% ) in groups B and C in August .On the whole ,there was higher similarity of all the samples in time than in space .The diversity of the flora showed that sediment microbial cloning in all treatment groups mainly belong to four phyla ,Proteobacteria (71% ) ,Verrucomicrobia (9% ) ,Actinobacteria(8% ) ,Chloroflexi (11% ) and other kinds of unknown microbes ,with dominant species Ideonella sp .,accounting for 24% .
		2015 Vol. (10): 621-628 [Abstract] ( 79 ) HTML (0 KB) PDF (5620 KB) ( 283 )

	629	Suitable Feeding Frequency of Juvenile Chinese Giant Salamander
		YANG Zhenjiang,XIAO Hanbing,TIAN Haifeng,MENG Yan,HU Qiaomu,WANG Fang
		Chinese giant salamander (Andrias davidianus) juveniles (18 months old) were reared in 193 cm × 167 cm cement tanks at a rate of 80 individuals per tank and fed in various feeding regimes at water tem‐perature of 19 ± 1 ℃ to evaluate the suitable feeding frequency via grow th performance ,body composition and digestive enzyme activity in an intensive aquaculture .The results showed that there were elevated food intake and digestive enzyme activity in the fasted salamander .The maximal weight gain rate (WGR) and specific growth rate (SGR) and minimal food conversion ratio (FCR) were observed in the salamander fed and fasted alternatively in a 6 day interval ,significantly higher or lower than those in the other groups(P<0.05) .The salamander fed and fasted alternatively in a 2 day interval(S2F2) had high protein content and low fat content ,and there was lower survival rate in the salamander fed and fasted alternatively in a 6 or 8 day interval (S6F6 and S8F8) than that in other groups .When very high survival rate and body nutri‐ents were hoped ,the suitable feeding frequency was of feeding and fasting alternatively in a 2 day interval . If not ,suitable feeding frequency was of feeding and fasting alternatively in a 6 day interval ,which the ju‐venile salamander had relatively high survival rate ,grow th performance and body nutrients .
		2015 Vol. (10): 629-633 [Abstract] ( 63 ) HTML (0 KB) PDF (672 KB) ( 295 )

	634	Changes in Shape and Nutrients in Decomposition of Tilapia Feces
		CHEN Jiajie,TAN Hongxin,YU Zhongli,LUO Guozhi,GAO Jinfang,XU Yaqian
		The bioflocculation process in a reactor was observed under a microscope and scanning electronic microscope and crude protein ,amino acids and extracellular polymeric substances were determined in feces of NEW GIFT Oreochromis niloticus collected in a recirculating aquaculture system .It was found that the feces became loosely ,feces developed into bioflocs framed with filamentous fungi with reaction of the bioflocs .With the decomposition of feces ,there was slow increase in level of the ammonium ,with the peak value of 7 .46 mg/L in the reactor on the 7th day ,and the nitrite was shown to be increased to 7 .81 mg/L , with the rapidly accumulative nitrate of 21 .6 mg/L .On the 9th day ,the crude protein had the peak content of 26 .37% ,and then decreased to 25 .45% ,and the total amino acids was found to be the peak value of 20 .44% on the 7th day ,and then decreased to 18 .11% .There were lower level of essential amino acids in bioflocs than in the feed (13 .03% ) , even though higher than in the feces (9 .36% ) . The polysaccharides were primariy substance in the loosely bound extracellular polymeric substances(LB‐EPS) in the bioflocs ,while protein was the main component in the tightly bound EPS(TB‐EPS)in the bioflocs .
		2015 Vol. (10): 634-639 [Abstract] ( 87 ) HTML (0 KB) PDF (3629 KB) ( 259 )

	640	Isolation,Identification and Antibiotic Sensitivity of Aeromonas veronii from Tilapia Cultured in Hainan Province
		LI Cong,CAI Yan,ZHOU Yongcan,YUAN Wei,CAO Ke,LIU Haitian,XIE Zhenyu,GUO Weiliang,WANG Shifeng
		There was an outbreak mortality of tilapia (Oreochromis niloticus) in several farms of Hainan province from the March to May ,2014 .Twelve dominant bacterial strains were isolated from diseased tila‐pia using routine bacterial isolation method .Artificial challenge test showed that the isolated strains HN‐G‐03 and HN‐B‐02 from the liver and brain of moribund tilapia possessed strong virulence ,the LD50 of the two strains to healthy tilapia by intraperitoneal injection challenge were 7 .12 × 105 cfu/fish and 1 .32 × 105 cfu/fish ,respectively .Based on the results of 16S rRNA and gryB gene sequence analysis ,morphological observation ,and physiological and biochemical characteristic analysis ,HN‐G‐03 was identified as Aero‐monas veronii bv . veronii and HN‐B‐02 was A . veronii bv . sobria . The antibiotic sensitivity analysis showed that both HN‐G‐03 and HN‐B‐02 were resistant to 12 kinds of drugs including penicillin ,furazoli‐done ,ampicillin ,spectinomycin ,cefuroxim ,and streptomycin ,only sensitive to levofloxacin ,doxycyc‐line ,florfenicol ,enrofloxacin and gentamicin .The findings provided a further scientific basis for the pre‐vention and treatment of tilapia diseases .
		2015 Vol. (10): 640-646 [Abstract] ( 65 ) HTML (0 KB) PDF (920 KB) ( 348 )

	647	Changes in Main Fish Stocks in Bohai Sea around Shandong Province
		LI Qiaochu,ZOU Yan,ZHANG Shaochun,WANG Yingjun,ZHAO Dan,SONG Aihuan
		T he changes in 10 common species of fish resources w ere analyzed in the Bohai Sea around Shan‐dong province based on the data (1996 -2012) of marine capture productions in Shandong province ,and the reason of the changes and the policy guarantee measures were discussed .The capture production in the Bohai Sea around Shandong province has been increased since 1996 ,and declined significantly since 2007 , with steady and diversified structure in fish stock .The stocks of soiuy Lizahaematocheila ,pomfret Pam‐pus argenteus and hairtail Trichiurus lepturus were declined notably due to excessive exploitation of ma‐rine living resources .Along with development of marine management ,stock of Japanese spanish mackerel Scomberomorus niphonius was stable ,and anchovy Engraulis j aponicas had been in severe recession since 2006 because of many year development in a large scale .
		2015 Vol. (10): 647-651 [Abstract] ( 87 ) HTML (0 KB) PDF (2061 KB) ( 552 )

	652	Culturable Bacterial Diversity in Intestine of Farmed Juvenile Puffer Fish Taki f ugu rubripes
		LI Yanyu,REN Meng,ZHANG Congyao,WANG Lianshun,ZHU Ying,MA Yuexin
		It w as aimed to examine the bacterial community associated to intestinal mucus of farmed juvenile puffer fish Takifugu rubripes .Polymerase chain reaction and sequencing of partial 16S rDNA were performed on DNA from bacteria cultivated on Zobell 2216E .All the isolates were classified into two phyla , Proteobacteria and Firmicutes . Gamma‐Proteobacterium comprised the dominant culturable intestinal microbiota (83 .3% ) ,and 8 genera of bacteria were isolated from the intestine ,including V ibrio , Shewanella,Pseudoalteromonas,Sulfitobacter,Bacillus,Aliivibrio,Photobacterium,and Colwelliain which Vibrio , Shewanella ,and Pseudoalteromonas accounted for 70% of the total . Sulf itobacter and Colwellia were the first reported as the intestinal bacterial microflora in puffer fish T .rubripes .
		2015 Vol. (10): 652-656 [Abstract] ( 56 ) HTML (0 KB) PDF (825 KB) ( 330 )

	657	Profile Characteristics of COD and Its Influencing Factors in Rushan Bay and Adjacent Area
		LIU Ying,ZHANG Xuechao,LI Xiaomin,SONG Jide,SONG Xihong,HU Hongzhi
		The profile characteristics of chemical oxygen demand (COD ) were studied based on the investigation data in Rushan Bay and adjacent area in spring (May) and autumn (October) of 2011 .The influencing factors and contribution of COD to eutrophication were also analyzed .The results showed that the distribution of COD was characterized by a decreasing tendency from the upper bay to the outside bay in spring and autumn .The concentration of COD was higher in spring than that in autumn .The content of COD in spring and autumn showed a negative correlation with temperature and salinity (P< 0 .01) ,while a positive correlation with level of dissolved inorgainic nitrogen(DIN) (P< 0 .05) .The range of COD contribution to the eutrophication was from 39 .53% to 67 .18% ,COD as an important factor contributing to eutrophication ,not the decisive factor .
		2015 Vol. (10): 657-661 [Abstract] ( 64 ) HTML (0 KB) PDF (979 KB) ( 462 )

	662	Research Progress on Assessment of Immune Effect of Fish Vaccines
		JIANG Xinyu,ZHANG Chao,LI Xudong,WANG Fei,KONG Xianghui

		2015 Vol. (10): 662-666 [Abstract] ( 48 ) HTML (0 KB) PDF (640 KB) ( 296 )