Quick Search
Office Online
Journal Online
Download
More...
Information
  • 月刊，1984年创刊
    主 管：内蒙古自治区教育厅
    主 办：包头医学院
    主 编：赵云山
    特邀主编：高长青
    编辑出版：包头医学院学报编辑部
    国际刊号：ISSN 1006－740X
    国内刊号：CN 15－1182/R
    邮发代号：16-292
Links
More...
2024 Vol. 43, No. 5
Published: 2024-09-25
Research and Application
Overview and Specific Topic
 
       Research and Application
683 Inhibition of TiLV Replication by AT2-R in Nile Tilapia Oreochromis niloticus
WEN Jing, ZHENG Shucheng, KE Zishan, WANG Yingying, LI Yingying, MO Xubing, ZHANG Defeng, YIN Jiyuan, ZHOU Wenli, WANG Qing
DOI: 10.16378/j.cnki.1003-1111.23012
In order to explore the function of type-2 angiotensin Ⅱ receptor (AT2-R) of Nile tilapia Oreochromis niloticus in the process of tilapia lake virus (TiLV) infection, the complete open reading frame (ORF) sequence of AT2-R gene of Nile tilapia AT2-R (OnAT2-R) was cloned by reverse transcription PCR (RT-PCR) and the tissue distribution and phase expression were analyzed in OnAT2-R by real-time fluorescence quantitative PCR (qRT-PCR). Meanwhile, the recombinant eukaryotic expression vector pEYFP-OnAT2-R was constructed for subcellular localization analysis in tilapia brain (TiB) cells which were infected with TiLV after overexpression of OnAT2-R gene, and the relative expression of TiLV-S8 segment was detected by qRT-PCR. The results showed that the OnAT2-R gene ORF sequence of the Nile tilapia was found to be 1338 bp in length, encoding 445 amino acids with a 7-transmembrane region. The OnAT2-R gene was found in all tissues examined in the Nile tilapia, with the maximal expression level in liver, followed by muscle and skin, and the minimal expression level in spleen and stomach. The relative expression level of OnAT2-R gene in the liver was shown to be increased first and then decreased in the Nile tilapia with TiLV infection, while the relative expression of OnAT2-R gene in the muscle was increased and then decreased, followed by increased again. Furthermore, the pEYFP-OnAT2-R was successfully constructed and the subcellular localization analysis showed that OnAT2-R protein was mainly localized on the cell membrane. The mRNA level of TiLV-S8 segment was significantly reduced in TiB cells overexpressed with OnAT2-R gene followed by TiLV infection. The finding indicates that OnAT2-R is a potential cell surface molecular receptor located on the cell membrane and has the effect on inhibition of TiLV replication, which will provide theoretical references with further in-depth research on pathogenesis of TiLV infection and antiviral mechanism of tilapia.
2024 Vol. 43 (5): 683-693 [Abstract] ( 165 ) HTML (1 KB)  PDF (14786 KB)  ( 51 )
694 Effects of Dietary Fresh Wolffia Wolffia globosa on Growth, Nutrition Quality and Intestinal Microbita of Common Carp Cyprinus carpio var. Quanzhounensis
WEN Luting, DU Xuesong, LI Zhe, WU Xia, HUANG Yin, QIN Junqi, HUANG Bo, LI Min, DENG Qian, LIN Yong, CHEN Zhong
DOI: 10.16378/j.cnki.1003-1111.21161
To investigate the effects of dietary fresh wolffia Wolffia globosa on the growth performance, nutritional composition, antioxidant capacity, gut microbiota structure, and digestive enzyme activity of common carp Cyprinus carpio var. Quanzhouensis, juvenile fish with body weight of (2.44±1.11) g was raised in a 6.55 m ×1.58 m×0.90 m aerated cement tank and fed 3% granular feed and 2% fresh wolffia daily at water temperature of (23.00±0.72) ℃ (experimental group), while in the control group the fish was fed 5% formulated feed per day for 12 weeks when the growth performance, nutritional composition, antioxidant capacity, intestinal microbiota structure, and digestive enzyme activity of the carp were measured.The results showed that there were very significant higher increase in body mass, specific growth rate, hepato-somatic index, and intestinal-somatic index in the common carp in the experimental group than those in the control group (P<0.01),without significant differences in condition factor, feed conversion ratio and gonado-somatic index between the two groups (P>0.05).The spleen-somatic index was significantly higher than those in the control group (P<0.05). Compared with the control group, the contents of crude protein and crude fat in the muscle of the common carp in the experimental group were increased significantly (P<0.01), while the contents of crude ash and cholesterol decreased significantly (P<0.01). The total antioxidant capacity of hepatopancreas(T-AOC) of the fish fed with fresh W. globosa were significantly higher than those of the control group (P<0.01). The W. globosa had no effect on the species diversity and abundance of intestinal microbiota of the common carp and the number of absolute dominant bacteria (P>0.05), but the relative abundance of enterococci positively related to crude fat and total antioxidant capacity were significantly increased ,while the relative abundance of campylobacter positively related to cholesterol and crude ash were significantly decreased, the functions of intestinal material degradation, metabolism, replication, repair and digestion were enhanced; while the activity of trypsin in foregut were increased (P<0.01). To sum up, all results suggest that dietary 2% fresh W. globosa can improve the degradation, metabolism and digestion functions of intestinal microbiota of the common carp, promote its trypsin activity and T-AOC, thereby improving its growth performance and the nutritional structure of muscle. W. globosa could be used as a high-quality biological feed for the fish fed in industrial and paddy field cultivation.
2024 Vol. 43 (5): 694-706 [Abstract] ( 107 ) HTML (1 KB)  PDF (5324 KB)  ( 35 )
707 Effects of Different Nutrient Levels on Gluconeogenesis and Glycogenolysis of Juvenile Mandarin Fish Siniperca chuatsi
MA Chenxi, ZHAO Jinliang, SONG Yindu, ZHAO Liangliang
DOI: 10.16378/j.cnki.1003-1111.22088
In order to explore the effects of three nutrient levels of normal feeding, glucose injection and starvation on the endogenous glucose production pathway of mandarin fish (Siniperca chuatsi), mandarin fish with body weight of (36.70±0.52) g were reared in cages of 1 m×1 m×1 m at stocking density of 25, 25 and 15 fish per box at water temperature of (23.0±0.5) °C, respectively, and were fed live grass carp Ctenopharyngodon idella with body weight of (8.15±0.16) g fingerlings (normal feeding group), 1 mg/g body weight intraperitoneal injection of glucose (glucose group) and starvation for 15 days (starvation group). Blood glucose and liver glycogen contents, insulin level, glucagon level, and activities of key enzymes in gluconeogenesis, including phosphoenolpyruvate carboxykinase (PEPCK), fructose-1,6-bisphosphatase (FBPase) , and glucose-6-phosphatase(G6Pase)were measured 1, 2, 4, 6, 12 and 16 h after feeding, 1, 2, 4, 6, 8, 12, and 24 h after injection, and 5, 10 and 15 days after starvation, and the relative mRNA expression levels was detected in each enzyme gene using real-time PCR. The results showed that blood glucose and liver glycogen contents and insulin level were significantly increased in the normal feeding group, and glucagon was significantly decreased, without significant change in the activity of key enzymes and inhibition of the activities of GPase, FBPase and GPase in gluconeogenesis. There were persistent hyperglycemia, and inhibition of PEPCK and the GPase activities and the relative expression levels of GPase in glucose injection group, without inhibition of the FBPase and G6Pase activities, and without significant changes in relative expression levels of PEPCK, FBPase, and G6Pase genes. The mandarin fish in starvation group had significant decrease in the blood glucose content in 10 days, and significant increase in glucagon content for 5 days, mainly relying on glycogenolysis to generate glucose from significantly elevated GPase activity during pre-and mid-stage (5 d and 10 d) of starvation. There were significant increase in relative expression levels of GPase, PEPCK, and G6Pase genes, thus glucose was mainly generated by gluconeogenesis at 15 day starvation. It was found that the level of blood glucose was significantly increased in the normal feeding group, and that the glycogenolysis pathway was significantly inhibited. The increase in 0—6 h blood glucose content was speculated to be caused by food digestion and absorption, with the peak of “hyperglycemia” at 6 h, and 6—16 h food digestion and absorption continue, after glucose absorption, glycogen synthesis was significantly improved, thus maintaining blood glucose balance. Due to high blood glucose levels, at the same time, glycogenolysis was inhibited (GPase activity decreased 0—12 h). The highest blood glucose level at 6 h reflected the time that the mandarin fish maintained "blood glucose balance". If the blood glucose level continued to rise, it caused physiological abnormalities in the body. The levels of blood glucose had different effects on gluconeogenesis and glycogenolysis pathways of mandarin fish. The gluconeogenesis and glycogenolysis pathways were regulated by the level of blood glucose.
2024 Vol. 43 (5): 707-716 [Abstract] ( 85 ) HTML (1 KB)  PDF (3728 KB)  ( 36 )
717 Feeding Habits of Hairtail Trichiurus japonicus in the Inshore Waters of Southern Zhejiang in Summer and Autumn
LIU Zhihao, HAN Dongyan, GAO Chunxia, YE Shen
DOI: 10.16378/j.cnki.1003-1111.21016
The feeding habits hairtail Trichiurus iaponicas were investigated in 599 samples in the inshore waters of southern Zhejiang province in summer and autumn of 2019 by stomach content analysis. Relationships between the feeding habits and environmental factors were evaluated by Canonical Correspondence Analysis (CCA). The results showed that the prey items of T. japonicus included more than 60 species, with dominant preys of brachyura megalopa larva, Benthosema pterotum and Euphausia pacifica. Obvious seasonal variations were revealed for T. japonicus's diet composition, with the percent index of relative importance (IRI%), pisces, planktonic larva, Isopoda and euphausiacea were higher in summer, and the IRI% of pisces and decapoda were higher in autumn. With the increase of T. japonicus's anal length, the main prey items were transferred from small size items (such as planktonic larva and small fish) to big size items (such as Larimichthys polyactis and T. japonicus). Besides, the results of CCA indicated that pH, sea surface temperature and sea surface salinity were the main environmental factors affecting the feeding habits of T. japonicus in the inshore waters of southern Zhejiang. The findings enriched the understanding of feeding habits of T. japonicus in the inshore waters of southern Zhejiang, and provided basic data for ecosystem-based fisheries management in this area.
2024 Vol. 43 (5): 717-726 [Abstract] ( 107 ) HTML (1 KB)  PDF (1361 KB)  ( 69 )
727 Optimization of Purification Process of Saponins from Sea Cucumber Apostichopus japonicus with Macroporous Resin by Response Surface Methodology
LIU Yujun, LIU Guiying, YU Di, LI Long, FU Zhiyu, ZHENG Jie, LIU Yutong, ZHOU Zunchun
DOI: 10.16378/j.cnki.1003-1111.23080
In order to optimize the optimum process conditions for purifying saponin Holotoxin A1 (HA1) by macroporous resin, four different structural macroporous resins (AB-8, HP-20, D101 and NKA-9) commonly used for purification of saponins in sea cucumber Apostichopus japonicus were selected. The content of HA1 was determined by high performance liquid chromatography (HPLC) using sea cucumber saponin HA1 as a standard. By comparing their static adsorption-desorption capacity and static adsorption curves, the suitable resin type was selected. The crude saponin mass concentration, ethanol volume fraction and ethanol washing volume were used as factors to determine the optimal dynamic purification process conditions based on the single factor experiments and Box-Behnken response surface experiment. The results showed that AB-8 resin had the best purification effect on HA1, and the optimal purification process was as follows: crude saponin mass concentration of 25 mg/mL, flow rate 1 mL/min, sample loading volume 25 mL, washing volume 2 times column volume (BV), ethanol volume fraction 73%, and ethanol washing volume 7 BV. Under these conditions, the recovery rate of HA1 was 91.40%, and the purity was increased from 0.16% to 0.58%. The purification process obtained in this study is suitable for the purification of saponin HA1 in Apostichopus japonicus.
2024 Vol. 43 (5): 727-736 [Abstract] ( 87 ) HTML (1 KB)  PDF (4743 KB)  ( 28 )
737 Differences in Morphology and Carbon and Nitrogen Contents of Venus Clam Meretrix meretrix from Different Geographical Populations
LIU Wei, HU Qingbiao, MEI Jie, FENG Jialing, MA Min, LI Xiaodong
DOI: 10.16378/j.cnki.1003-1111.23036
In order to explore the differences in morphology and carbon and nitrogen contents of different geographical populations of venus clam Meretrix meretrix, morphological traits including shell length, shell width and shell height were measured in 180 sample of venus clam collected from Binzhou, Shandong (E 118°02', N 37°22'), Rudong, Jiangsu(E 121°18', N 32°33') and Geli-gang, Liaoning (E 121°97', N 40°80'), and the correlation was analyzed between carbon and nitrogen contents of shell and soft tissue and morphological traits and body weight by correlation analysis, path analysis and multiple regression analysis. The results showed that there were high proportion of carbon and nitrogen contents in most of the clam in the three geographic populations, with high proportion of carbon and nitrogen contents in the shell and mollusk tissues. The most morphological traits were shown to be significantly correlated with body weight in the three geographic populations (P<0.05), with the maximal correlation of shell width with body weight, with correlation coefficient of 0.919, 0.640, and 0.696, respectively (P<0.01). The pass analysis and coefficient of determination analysis revealed that shell width, shell length, and shell height were the main morphological traits affecting the body weight of clams in Binzhou, Rudong, and Geli-gang populations, respectively. By testing the significance of partial regression coefficients, the optimal regression equations of morphological traits on body weight in the three groups were established, and the carbon sequestration capacity of clam shell and clam soft tissues was higher in the geographic group of Geli-gang in Liaoning than that in the groups of Binzhou in Shandong and Rudong in Jiangsu. The findings provide references with morphological discrimination of geographic populations of the clam, analysis of germplasm resources and research on low-carbon sustainable fisheries.
2024 Vol. 43 (5): 737-745 [Abstract] ( 95 ) HTML (1 KB)  PDF (5545 KB)  ( 41 )
746 Molecular Phylogenetic Relationship in Grouper Species in Genus Epinephelus Based on Sequence of ND1 Gene
HE Haobin, ZHANG Guoqing, GAO Zihan, HU Kaihong, LIN Jiaxin, ZHOU Meng, HUANG Yanhua, LIANG Rishen
DOI: 10.16378/j.cnki.1003-1111.22071
In order to analyze the taxonomic relationship of grouper species in genus Epinephelus in China at molecular level and clarify the taxonomic controversy, the molecular phylogenetic relationship was comprehensively analyzed.in 91 samples of 37 species collected in aquatic product markets, fishing ports and wharves in coastal provinces, cities or regions such as Guangdong, Fujian, Hainan, Zhejiang, Hong Kong, and even Australia from 2019 to 2021 based on NADH dehydrogenase subunit I (ND1) gene. The result showed that the consensus ND1 gene sequences were determined to be 645 bp, with genetic distance from 0.0062 to 0.2636, and the average value of 0.1547 among species. The molecular phylogenetic trees were constructed using Cephalopholis species as outgroup, and 37 species of groupers were divided into two parallel clades Epinephelus in the tree, most species relationships with clear and high noded supports. Certain grouper pairs:E. morrhua/E. radiatus, E. maculatus/E. bilobatus, and E. coeruleopunctatus/E. ongus, with taxonomic controversies, were tightly clustered together, but the branch lengths clearly distinguished each pair of groupers. There was larger than the value 2% of genetic distances in ND1 gene in each pair of groupers, indicating that groupers in each pair were distinct species. Within the pair of groupers E. flavocaeruleus and E. cyanopodus, the ND1 genetic distance between them was 0.0062, and their branch lengths were extremely short and hard to be distinguished, revealing that they might be the same species in molecular level.The findings indicated that the molecular phylogenetic relationship among 37 species in genus Epinephulus was developed and that the synonymous controversies of certain species with similar morphological characteristics are clarified, which provides the molecular evidences for the classification of species in genus Epinephulus.
2024 Vol. 43 (5): 746-756 [Abstract] ( 71 ) HTML (1 KB)  PDF (1481 KB)  ( 33 )
757 Flocculation and Fragmentation of Particulate Matter in Biofloc Culture Water by Ozone
CHENG Xiangyu, LIU Xingguo, CHEN Zhe, XIAO Shuwen, PAN Hengfeng, ZHANG Wang
DOI: 10.16378/j.cnki.1003-1111.23033
In order to understand the effect of ozone on flocculation and fragmentation of suspended particulate matter in aquaculture wastewater, the ozone concentrations corresponding to 0.5, 1.0, 1.5, 2.0 and 2.5 mg/L were aerated by an ozone generator for 5, 10, 15, 20 and 25 s in a 1 L ozone reaction vessel at a water temperature of (29±1) °C, respectively. Each ozone concentration was oxidized with the raw water in the circulating water biofloc shrimp culture system in Sanmao Aquaculture Farm in Songjiang District, Shanghai for 1, 2, 3, 4 and 5 min, and each combination test was repeated 3 times. Immediately after the treatment, the water quality index, the concentration of total residual oxidant (the concentration of total residual oxidant determined immediately at the end of aeration) and the particle size distribution of suspended particulate matter were measured in the water samples.The results showed that different ozone concentration treatments caused significantly increase in the levels of ammonia nitrogen (NH+4-N) and nitrate nitrogen (NO-3-N) contents in the water (P<0.05),with significant decrease in nitrite nitrogen (NO-2-N) level in the water column (P<0.05). There was significant decrease in total suspended solids (TSS) in the water (P<0.05) with different concentrations of ozone treatment, and the gradual increase in the removal rate of TSS with the elevated ozone concentration, with the maximum removal rate of 23.15% at ozone concentration of 2.5 mg/L. The size distribution of suspended particles in the water was found to be changed significantly after the ozone was put into the culture water, with gradual increase in the flocculation efficiency with the increase in ozone concentration, and the maximum flocculation efficiency of 33.61% at the ozone concentration of 2.5 mg/L.The findings indicated that ozone effectively removed nitrite nitrogen and flocculated suspended particles in the water.It is recommended that treatment of aquaculture waterbe conducted at the suitable ozone concentration of 1.5 mg/L for 4 min.
2024 Vol. 43 (5): 757-766 [Abstract] ( 65 ) HTML (1 KB)  PDF (1111 KB)  ( 29 )
767 Comparative Analysis of Intermuscular Bones of Red Common Carp Cyprinus carpio var. wuyuanensis, Crucian Carp Carassius auratus var. pengsenensis, and C. auratus var. pingxiangnensis
ZHANG Qin, WANG Hong, CHEN Yu, ZHAO Daxian, SHENG Junqing, ZHANG Wanchang
DOI: 10.16378/j.cnki.1003-1111.23019
Red common carp Cyprinus carpio var. wuyuanensis, crucian carp Carassius auratus var. pengsenensis and C. auratus var. pingxiangnensis were put into a pot and boiled, then the intermuscular bones were removed, and the number, morphology, type and distribution of the intermuscular bones were compared and analyzed by conventional measurement and anatomical methods.The results showed that there were intermuscular bones ranging from 70 to 99 in red common carp, with an average of 88, ranging from 78 to 92 in crucian carp, with an average of 85, and ranging from 62 to 89 in C. auratus var. pingxiangnensis, with an average of 77, without significant difference in the numbers of intermuscular bone between red common carp and crucian carp, and with significant differences in the numbers of intermuscular bone between red common carp and C. auratus var. pingxiangnensis(P<0.01).There were epineuria bones and epipleural bones in intermuscular bones among all the three fish varieties, without epicentraliabones. The simple type (″ I ″type,″ 卜 ″type,″ Y ″type), middle type (one end multi-fork, two ends two-fork) and complex type (two ends multi-fork, and twig-shaped type) were found in intermuscular bones, with the most simple types. The numbers of intermuscular bones on both sides of the body were not completely consistent, and the distribution of intermuscular bone types was different in different positions among the three fish warieties, and the distribution of intermuscular bones was different on the left and right sides, without significant difference. The findings provide basic data with further research on the spatiotemporal development of intermuscular bone and breeding new strains of cyprinids with little or no intermuscular bone.
2024 Vol. 43 (5): 767-774 [Abstract] ( 83 ) HTML (1 KB)  PDF (2453 KB)  ( 41 )
775 Denitrifying Microorganisms on Suspended Carrier Biofilm in a Recirculating Aquaculture System of Pacific White Shrimp Litopenaeus vannamei
LI Yayuan, ZHANG Wang, LIU Xingguo, XIAO Shuwen, JIANG Haixin, LU Shimin
DOI: 10.16378/j.cnki.1003-1111.23010
To understand the nitrate reduction potential and nitrogen metabolism pathway character of moving bed bioreactor in a Pacific white shrimp Litopenaeus vannamei in a recirculating aquaculture system(RAS), the microbial community structure and denitrification efficiency of biofilms were analyzed on the surface of suspended carriers. Illumina sequencing results revealed that Proteobacteria(20.31%—25.36%) and Bacteroidetes(14.42%—24.28%) were dominant in all samples. At the genus level, aerobic denitrifiers such as Rhodococcus (0.28%—0.97%), Paracoccus (0.50%—0.99%) and Pseudomonas (0.15%—0.30%) were primarily responsible for nitrate reduction. Furthermore, qRT-PCR detection showed that the denitrification functional gene nirK was more abundant than that of nirS and nosZ, so the predominant denitrification bacteria were of the nirK-type.The prediction results from functional annotation of prokaryotic taxa (FAPROTAX) confirmed the presence of active heterotrophic denitrification in biofilms, the denitrifying bacteria were mainly competitive with each other and with the dominant biofilm bacteria,possibly due to the limited carbon sources. The anaerobic nitrogen removal tests showed that there was nitrate removal efficiency of up to 98.07%, without adding any carbon source. Based on the characteristics of nitrogen metabolism pathway, the partial denitrification-anammox process for shrimp aquaculture wastewater nitrogen removal was further proposed. The finding provides novel insights into comprehending of nitrogen metabolic pathways in RAS and future management, utilization of endogenous carbon sources in the biofilms, and potential denitrification functions.
2024 Vol. 43 (5): 775-783 [Abstract] ( 75 ) HTML (1 KB)  PDF (12051 KB)  ( 22 )
784 Inhibition Effect of EM Bacteria on Cyanobacteria
ZHOU Yongjia, LIU Xingguo
DOI: 10.16378/j.cnki.1003-1111.23042
In order to evaluate the inhibitory effect of EM bacteria on cyanobacteria, EM bacteria including Lactobacillus plantarum, Acetobacter tropicalis, and Bacillus velezensis solution with total density of viable bacteria of 1×108 cfu/L were added to the cyanobacteria including Microcystis(content>95%), Anabaena, Oscillatoria and Spirulina solution at a dose of 0%(control group), 0.40%, 0.80%, 1.60% and 3.20% (volume fraction), and the concentrations of dissolved total nitrogen, dissolved total phosphorus, and dissolved organic carbon, pH, cyanobacteria density and total chlorophyll a level in the solution were determined at different culture periods. The results showed that adding 0.80%, 1.60% and 3.20% EM bacterial solution led to significantly reduce the concentration of total dissolved nitrogen in solution (P<0.05). The concentration of dissolved total phosphorus in the solution was shown to be significantly decreased by adding 3.20% of EM bacterial solution in the first 3 days (P<0.05), with little effect on the concentration of dissolved organic carbon in the final solution. There was stable pH in the solution containing EM bacteria. EM liquid was found to be significant inhibitory effect on cyanobacteria (P<0.01), with inhibitory rate of up to 90.00%, and the order of inhibitory effect as 0.80%>3.20%>1.60%>0.40%. The concentration of total chlorophyll a in solution was decreased by adding EM bacterial solution, with the descending order effects of inhibitory effect were as 0.80%>1.60%>0.40%>3.20%, 0.80% and significant effects on the concentration of total chlorophyll a in solution in 1.60% of EM bacterial solution (P<0.05).In summary, the best effect on inhibiting cyanobacteria and reducing total chlorophyll a was observed in 0.80% EM bacterial solution which was used to control cyanobacteria bloom in natural water or aquaculture water. The finding provides reference for controlling cyanobacteria bloom outbreak and regulating algal phase structure in aquaculture water.
2024 Vol. 43 (5): 784-792 [Abstract] ( 114 ) HTML (1 KB)  PDF (1243 KB)  ( 83 )
793 Fishery Biological Characteristics of Reproductive Stocks of Estuarine Tapertail Anchovy Coilia mystus in Northern Hangzhou Bay
WANG Miao, HONG Bo, ZHANG Dan, YAN Jun
DOI: 10.16378/j.cnki.1003-1111.23039
In order to determine the composition and fishery biological characteristics of reproductive stocks of estuarine tapertail anchovy Coilia mystus in Northern Hangzhou Bay,samples of estuarine tapertail anchovy were collected by set net during the reproductive period from May to August 2018, 2019, and 2020. The fishery biological characteristics of estuarine tapertail anchovy including the total length, body weight, and sex ratio were investigated. The results showed that total length distribution of female reproductive stocks of estuarine tapertail anchovy in Northern Hangzhou Bay ranged from 113 to 233 mm, and two dominant groups were found at 130—150 mm and 170—200 mm (73.2%). The total length distribution of male reproductive stocks of estuarine tapertail anchovy in Northern Hangzhou Bay ranged from 88 to 204 mm, and the dominant group were found at 110—140 mm (66.0%). The body weight distribution of female reproductive stocks ranged from 3.6 to 29.7 g, and two dominant groups were found at 6.0—10.0 g and 14.0—20.0 g (64.2%). The body weight distribution of male reproductive stocks ranged from 1.3 to 16.2 g, and the dominant group were found at 2.0—8.0 g (90.0%). The relationship between total length (L) and body weight (m) of female reproductive stocks was described as m =0.518×10-5 L2.874(r2=0.978, n =984). The relationship between total length and body weight of male reproductive stocks was m =1.033×10-5 L2.692( r2=0.957, n =661). The sex ratio of female to male was 1.49∶1, and the number of female individuals was significantly higher than that of male individuals (P<0.05). The gonadal development of female individuals was mainly in stages Ⅳ and Ⅴ, and total length of individuals whose gonad maturity stages had achieved Ⅳ or above were mainly founded at 140—150 mm and 170—200 mm. The gonadal development of male individuals was mainly in stages Ⅲ and Ⅳ, and total length of individuals whose gonad maturity stages had achieved Ⅳ or above were mainly founded at 110—150 mm. The feeding levels of the reproductive stocks of estuarine tapertail anchovy was mainly 0 and 1, accounting for 95.8%, and the empty stomach rate was only 69.2%. The findings indicated that the female reproductive stocks of estuarine tapertail anchovy in Northern Hangzhou Bay consisted of several generations, and maintained a certain feeding level during the reproductive period.
2024 Vol. 43 (5): 793-800 [Abstract] ( 68 ) HTML (1 KB)  PDF (1099 KB)  ( 25 )
801 Effects of Salicylic Acid on High Temperature Resistance of Economic Brown Alga Sargassum thunbergii
WANG Xiaowei, LYU Fang, ZHOU Gefei, WU Haiyi
DOI: 10.16378/j.cnki.1003-1111.22067
In order to investigate the response of brown alga Sargassum thunbergii to high temperature stress and the effect of salicylic acid on its resistance to high temperature stress, four wild alga strains were placed in a 1000 mL conical flask, and 0, 1, 5, and 50 mg/L salicylic acid (SA) were added at 30 ℃ (treatment group), respectively. The growth rate, photosynthetic pigment content, chlorophyll fluorescence parameters of the alga strain were determined and the antioxidant system and HSP70 gene expression was detected after being cultured at high temperature for 3 days at 15 ℃ (control group) and 30 ℃ at different concentrations.The results showed that SA treatment significantly promoted the growth of the brown alga under high temperature. There were increase first and then decrease in specific growth rate, and chlorophyll a, chlorophyll c and carotenoid contents with elevated SA concentration, with the the most significant growth promotion in 5 mg/L SA group, with 589% increase in specific growth rate compared to the high temperature treatment, increase in the chlorophyll fluorescence parameter Fv/Fm, rebounded in non-photochemical quenching (NPQ) significantly, and increase in the activities of superoxide dismutase(SOD) and catalase (CAT). The expression level of HSP70 gene was found to be significantly increased under high temperature, 1 mg/L SA further promoting the expression of the HSP70 gene, whereas 5 mg/L and 50 mg/L SA leading to reduce HSP70 gene expression. The findings provide theoretical reference with the study of large-scale artificial breeding and mechanisms of stress resistance in S. thunbergii.
2024 Vol. 43 (5): 801-807 [Abstract] ( 68 ) HTML (1 KB)  PDF (1097 KB)  ( 32 )
808 Establishment of Primary Cell Culture Technique for Liver Tissue of Mandarin Fish Siniperca chuatsi
LUO Hanyu, LIU Xiao, ZHENG Feifei, MAO Tianyu, FENG Keyao, SUN Yanhong, ZHU Dongmei
DOI: 10.16378/j.cnki.1003-1111.23040
The primary cell culture of liver tissue of mandarin fish Siniperca chuatsi with body weight of(33.2±0.2) g was carried out by tissue explants adherent method, enzyme digestion method, and tissue explants adherent combined with enzyme digestion method for screening of the optimal method. The primary cell culture of liver tissue of mandarin fish was in 3 kinds of culture media (M199 culture medium, L15 culture medium, and M199-L15 culture medium), three kinds of culture containers (12 well plate, 6 well plate, and T25 culture bottle) and with or without CO2 were compared to determine the optimal culture conditions of primary cells from liver tissue of mandarin fish. The cells were identified using mitochondrial 16S rRNA gene sequences and chromosome count. The effects of ammonium chloride (0 mmol/L, 1 mmol/L, 5 mmol/L, and 10 mmol/L) on cell morphology and proliferation were also investigated. The results showed that the good cells of the liver tissue of mandarin fish was obtained by 0.25% trypsin-EDTA digested cells under conditions of M199-L15 (volume ratio 1∶1) mixed medium containing 20% fetal bovine serum, which were inoculated into T25 culture flask, and culture at 28 ℃ in an incubator containing 5% CO2. The attached cells accounted for 25%—30% of the bottom of culture flask at 2—3 days, the cells grew 70%—80% of the bottom of culture flask at 4—5 days, and primary monolayer cells were obtained at 6—7 days. The cells were plump and had strong proliferative ability after subculturing, appearing in a mixture of epithelioid and fibroblast-like cells, with fibroblast-like cells being the majority. The liver cells had grown up with 10 passages. The result of mitochondrial 16S rRNA gene sequencing was 99.05% consistent with the gene sequence in GenBank (HQ731435.1), and chromosome count was found to be 48, proving that these cells were from mandarin fish. The significant effects on cell morphology and vitality (P<0.05) were observed at 5 mmol/L and 10 mmol/L ammonium chloride exposure. The primary cell culture technology of liver tissue of mandarin fish was established, and provided an important basis with the development of biology, disease, nutrition and genetics of mandarin fish, and primary culture and establishment of other fish liver cell lines.
2024 Vol. 43 (5): 808-816 [Abstract] ( 62 ) HTML (1 KB)  PDF (5758 KB)  ( 41 )
817 Population Structure Analysis of Loach Barbatula nuda in Eastern Liaoning Province
DUAN Youjian, LUO Xiaonian, KANG Yi, LI Jiao, WU Chen, ZHANG Zhiming, SUN Shuhao
DOI: 10.16378/j.cnki.1003-1111.23035
In order to explore the population structure of loach Barbatula nuda in eastern Liaoning, the total length, body weight, age and first maturity performance were investigated in 161 samples collected from the tributaries of Yalu River and the upper reaches of Taizi River from 2018 to 2019 by a 1.5 cm mesh size trap to probe the population structure with vertebrae as the identification material. The results showed that the females had total length from 62 mm to 124 mm, and body weight from 1.7 g to 13.6 g, and that the males had body length from 71 mm to 130 mm, and the body weight from 2.1 g to 14.6 g, with age composition of 1—3 a and ratio of male to female of 0.916∶1. The estimation results of total length (TL) 50% method showed that the female was first sexual maturity at total length of 79.3 mm, and the male was first sexual maturity at total length of 98.9 mm, corresponding to the first sexual maturity age of 0.69 a and 1.19 a, respectively. In conclusion, B. nuda is a fish with small size, simple age composition and early sexual maturity, and the ecological strategy of the population is an r selection type. Overfishing will lead to deficiency in the population recovery of B. nuda and the decline of population resources, although the population recovery of this fish is fast when suffering from exploitation. Therefore, it is necessary to strengthen fishery management measures, such as setting up the minimum fishing specifications, domestication, breeding and releasing activities for conservation.
2024 Vol. 43 (5): 817-821 [Abstract] ( 99 ) HTML (1 KB)  PDF (3002 KB)  ( 37 )
       Overview and Specific Topic
822 A Review: Application and Prospect of Fish Rheotaxis in a Recirculating Aquaculture System
ZHOU Yinxin, LIU Haibo, HU Wei, REN Xiaozhong, LI Meng
DOI: 10.16378/j.cnki.1003-1111.23005
2024 Vol. 43 (5): 822-832 [Abstract] ( 102 ) HTML (1 KB)  PDF (1088 KB)  ( 58 )
833 A Review of Feeding Ecology of Jumbo Squid Dosidicus gigas
HU Guanyu, ZHAO Zhenfang, CHEN Xinjun, LIANG Jiawei, LIU Bilin
DOI: 10.16378/j.cnki.1003-1111.21111
2024 Vol. 43 (5): 833-842 [Abstract] ( 85 ) HTML (1 KB)  PDF (1083 KB)  ( 38 )
843 Research Progress on Toxic Effects of Pyrethroids Insecticides on Cultured Crustaceans: a Review
LUAN Keer, XIAN Jian'an, LU Yaopeng, ZHANG Zelong, ZHANG Xiuxia, LI Juntao, ZHENG Peihua, WANG Qian
DOI: 10.16378/j.cnki.1003-1111.23043
2024 Vol. 43 (5): 843-852 [Abstract] ( 90 ) HTML (1 KB)  PDF (1053 KB)  ( 109 )
Copyright © 2017 Editorial Board of Fisheries Science
Support by Beijing Magtech