Isolation and Identification of Bacterial Pathogen Aeromonas hydrophila and Development of the Inactivated Vaccine in Japanese Flounder Paralichthys olivaceus Diseased with Ascides
SUN Yi-min,LI Nan,SONG Jie,LI Xiao-yue,SHEN Hong-qi,WANG Feng-min,LI Quan-zhen,CAO Jie-ying,ZHAO Bao-hua
Abstract：The pathogenic bacteria were isolated from diseased Japanese flounder Paralichthys olivaceus, and identified as Aeromonas hydrophila by biochemical methods and polymerase chain reaction based on the 16s rDNA sequence on GenBank via the homologies of nucleic acids of 99.8% between the isolated strain and the standard strains. The mice and the flounder were immunized with the inactivated vaccine made by this isolated Aeromonas hydrophila via dipping immersion and showed protection rate of 90% and 80% with the incidence of 6%. The antibody level was detected by agglutination reaction and indirect enzyme-linked immunosorbent assay (ELISA) with the antiserum seemed immunogenic crucially. The anatomized results showed that the immunized mice were healthy and the unimmunized mice (as the control) were diseased in liver, indicating that the vaccine was effective.
收稿日期: 2009-11-25
引用本文:
孙祎敏,李楠,宋杰,李晓玥,申红旗,王风敏,李全振,曹杰英,赵宝华. 褐牙鲆腹水症病原菌的分离鉴定及其灭活疫苗的研制[J]. 水产科学, 2009, 28(11): 613-617.
SUN Yi-min,LI Nan,SONG Jie,LI Xiao-yue,SHEN Hong-qi,WANG Feng-min,LI Quan-zhen,CAO Jie-ying,ZHAO Bao-hua. Isolation and Identification of Bacterial Pathogen Aeromonas hydrophila and Development of the Inactivated Vaccine in Japanese Flounder Paralichthys olivaceus Diseased with Ascides. Fisheries Science, 2009, 28(11): 613-617.
