Abstract：To isolate the bioluminescence specific expressed genes of dinoflagellate Alexandrium catenella(A. catenella), suppression subtractive hybridization was performed to construct the differential expressing cDNA libraries of Alexandrium catenella. A total of 500 cDNA clones were picked. PCR amplification revealed that the length of inserted fragments was mainly from 250 to 1000 base pairs. Ten randomly selected clones after PCR screening were sequenced and analysed in GenBank with Blast search. Two ESTs shared significant identity with the luciferin binding protein and Ribulose-1,5-bisphosphate carboxylase/oxygenase and the other ESTs represent novel genes of Alexandrium catenella with no significant homology in GenBank. These results had provided the foundation for cloning new specific genes of Alexandrium catenella and further studying and establishing the novel detection method for specific dinoflagellata.
收稿日期: 2009-11-25
引用本文:
史西志,刘兵,贺静静,王梦前,郭皓,苏秀榕,李太武. 链状亚历山大藻抑制消减杂交文库的构建及分析[J]. 水产科学, 2009, 28(11): 675-677.
SHI Xi-zhi,LIU Bing,HE Jing-jing,WANG Meng-qian,GUO Hao,SU Xiu-rong,LI Tai-wu. Construction and Analysis of Suppression Subtractive Hybridization Library of Dinoflagellate Alexandrium catenella. Fisheries Science, 2009, 28(11): 675-677.
