Abstract:Females with initial body weight of (19.81±3.90) g and male with initial body weight of (18.55±3.65) g of Nile tilapia Oreochromis niloticus were fed basic diet containing 0 mg/kg (control group), 40 mg/kg, 80 mg/kg, 120 mg/kg and 160 mg/kg vitamin E at water temperature of (28.0±0.5) ℃ for 30 d. Then HE staining technique was used to make gonad and intestinal histological sections of female and male tilapia under different vitamin E concentrations to investigate the effects of dietary vitamin E on the reproductive performance, growth coefficient and intestinal morphology of female and male Nile tilapia. The results showed that the female tilapia fed the diet containing 120 mg/kg of vitamin E had the significantly higher intestinal villus height, the best growth performance and ovarian maturity. The male Nile tilapia fed the diet containing 160 mg/kg of vitamin E had the best growth performance and the best developed testis. There was no significant effect of supplementation of vitamin E on intestinal morphology of male Nile tilapia (P>0.05). The findings indicated that appropriate amount dietary vitamin E led to promote the gonad development of female and male Nile tilapia, the reproductive performance, the morphology and structure of intestinal tissue, and digestion and absorption of nutrients, which not only provided theoretical basis for the histological research of tilapia, but also provide a strong support for the healthy and sustainable development of the tilapia industry in the future.
张旭, 唐瞻杨, 黄凯, 王丽群, 宿志健, 刘婷, 王婧杰, 麻艳群. 维生素E对尼罗罗非鱼生长和性腺发育影响的研究[J]. 水产科学, 2020, 39(6): 887-893.
ZHANG Xu, TANG Zhanyang, HUANG Kai, WANG Liqun, SU Zhijian, LIU Ting, WANG Jingjie, MA Yanqun. Effects of Dietary Vitamin E on Growth and Gonadal Development in Nile Tilapia Oreochromis niloticus. Fisheries Science, 2020, 39(6): 887-893.
[1]肖登元,梁萌青.维生素在亲鱼营养中的研究进展[J].动物营养学报,2012,24(12):2319-2325. [2]蒋焕超.VE与硒的交互作用对吉富罗非鱼生长、生殖、抗氧化及免疫力的影响[D].南宁:广西大学,2012. [3]贾伟华,葛玲瑞,毛宏祥.维生素E在水产动物中的研究进展[J].江西水产科技,2017(5):40-43. [4]常杰,牛化欣,胡宗福,等.细鳞鲑幼鱼对饲料中维生素E的需求量[J].动物营养学报,2017,29(11):4085-4092. [5]张志强,蒋明,文华,等.胭脂鱼幼鱼对饲料中维生素E需要量的研究[J].西北农林科技大学学报:自然科学版,2017,45(2):23-30. [6]金明昌,汪开毓.水产动物的维生素E营养研究进展[J].淡水渔业,2006,36(4):59-61. [7]谢嘉华,杨儒明,陈雅瑜.维生素C和E对金鲫生长及繁殖的影响[J].泉州师范学院学报,2006,24(4):84-90. [8]覃希,黄凯,程远,等.维生素E和硒对吉富罗非鱼生殖激素及免疫功能的影响[J].饲料工业,2013,34(24):10-15. [9]杜雪松,林勇,梁国琨,等.两种罗非鱼的耐寒性能比较[J].广西师范大学学报:自然科学版,2019,37(3):174-179. [10]刘承英,赵燕,刘娟.斑马鱼石蜡组织切片技术的优化[J].安徽农学通报,2019,25(9):67-68. [11]郑伟,严继舟.斑马鱼组织石蜡切片质量的优化[J].江苏农业科学,2013,41(11):260-263. [12]樊佳伟,吴廷昌,陈猛猛,等.斜带石斑鱼(♀)×鞍带石斑鱼(♂)受精细胞学的组织切片观察[J].生命科学研究,2016,20(1):25-28. [13]王宁军,赵燕,刘娟.斑马鱼器官石蜡组织切片制作与观察[J].解剖学研究,2019,41(3):240-241. [14]郑尧,赵志祥,史磊磊,等.芦丁对吉富罗非鱼幼鱼组织切片及细胞凋亡的影响[J].淡水渔业,2017,47(3):73-78. [15]方珍珍,苏文,陶秉春.感染小瓜虫草鱼幼鱼组织病理学变化[J].水产科学,2015,34(7):433-437. [16]刘奕,赵婷婷,胡隐昌,等.布氏罗非鱼性腺发育的组织学研究[J].江西农业大学学报,2019,41(2):340-346. [17]王源,郝佳,王新权,等.饵料中添加不同含量水平的维生素E对三文鱼生产性能的影响[J].现代畜牧科技,2019(1):5-7. [18]刘汉鹏.七彩神仙鱼(Symphysodon haraldi)幼鱼饵料中最适维生素C、维生素E添加量的研究[D].上海:上海海洋大学,2018. [19]Lin Y H, Shiau S Y. Dietary vitamin E requirement of grouper, Epinephelus malabaricus, at two lipid levels, and their effects on immune responses[J]. Aquaculture,2005,248(1/2/3/4):235-244. [20]Lu Y, Liang X P, Jin M, et al. Effects of dietary vitamin E on the growth performance, antioxidant status and innate immune response in juvenile yellow catfish (Pelteobagrus fulvidraco)[J]. Aquaculture,2016,464:609-617. [21]张敏,黄藏宇,孙艳发,等.日粮维生素E水平对肉鸡屠宰性能和血脂代谢的影响[J].湖北农业科学,2016,55(8):2049-2052. [22]伍曦,罗辉,冯琳,等.高水平维生素E对幼建鲤生长性能和消化吸收功能的影响[J].动物营养学报,2011,23(11):1938-1945. [23]米海峰,孙瑞健,张璐,等.鱼类肠道健康研究进展[J].中国饲料,2015(15):19-22. [24]朱传忠,孙陆宇,解文丽,等.3种饲料添加剂对吉富罗非鱼幼鱼生长性能、消化酶活性和肝、肠组织结构的影响[J].淡水渔业,2018,48(2):94-99. [25]Liu W, Wu J P, Li Z, et al. Effects of dietary coated protease on growth performance, feed utilization, nutrient apparent digestibility, intestinal and hepatopancreas structure in juvenile gibel carp (Carassius auratus gibelio)[J]. Aquaculture Nutrition,2018,24(1):47-55. [26]王坛,赵金鑫,刘东来,等.饲用溶菌酶对吉富罗非鱼消化道组织结构和营养物质消化吸收的影响[J].渔业科学进展,2019,40(6):76-87. [27]胡海滨,刘金桃,李彦先,等.饲料中大豆黄酮对大菱鲆生长、消化酶活力、抗氧化力及肠道结构的影响[J].水产学报,2014,38(9):1503-1513. [28]张国辉,何瑞国,张世萍,等.维生素E对黄鳝繁殖性能的影响[J]. 华中农业大学学报,2006,25(2):177-181. [29]肖登元,梁萌青,王新星,等.饲料中添加不同水平的维生素E对半滑舌鳎(Cynoglossus semilaevis)亲鱼繁殖性能及后代质量的影响[J].渔业科学进展,2015,36(2):125-132. [30]李铭,董卫军,徐加元,等.维生素E对克氏原螯虾生殖的影响[J].水产学报,2007,31(增刊):65-68.